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Article
Significance of urinaryN 1,N 8-diacetylspermidine andN 1,N 12-diacetylspermine as indicators of neoplastic diseases
N 1,N 8-Diacetylspermidine (Ac2Spd) andN 1,N 12 diacetylspermine (Ac2Spm), the occurrence of which in healthy human urine was demonstrated recently, increased much more frequently and markedly than total polyami...
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Article
Diagnostic and prognostic usefulness of N 1,N 8-diacetylspermidine and N 1,N 12-diacetylspermine in urine as novel markers of malignancy
Recently, we found N 1,N 8-diacetylspermidine (Ac2Spd) and N 1,N 12-diacetylspermine (Ac2Spm) in human urine, and noted that their amount increased significantly in patients with urogenital malignancies. Previou...
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Article
Synthesis of 2-Methyl 16-Membered Macrolide Derived from Tylosin
To improve the metabolic stability of a 16-membered macrolide, 2-methylated derivatives of desmycosin were synthesized. Among these derivatives, 2β-methyldesmycosin retained antibacterial activity and showed i...
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Article
The role of glutathione on the cytotoxic effects and cellular uptake of diphenylarsinic acid, a degradation product of chemical warfare agents
The mechanism underlying enhancement of the cytotoxic effects of diphenylarsinic acid (DPAA) by sulfhydryl (SH) compounds, such as glutathione (GSH) and dimercaptopropane sulfonate (DMPS), was investigated in ...
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Article
Reliable and Fast Allele-Specific Extension of 3′-LNA Modified Oligonucleotides Covalently Immobilized on a Plastic Base, Combined with Biotin-dUTP Mediated Optical Detection
In the present work, a convenient microarray SNP ty** system has been developed using a plastic base that covalently immobilizes amino-modified oligonucleotides. Reliable SNP allele discrimination was achiev...
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Article
Pharmacokinetics of arsenic species in Japanese patients with relapsed or refractory acute promyelocytic leukemia treated with arsenic trioxide
To investigate the pharmacokinetics of arsenic species in Japanese patients with relapsed or refractory acute promyelocytic leukemia (APL) treated with arsenic trioxide (ATO) at a daily dose of 0.15 mg/kg.
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Article
Adsorption of 4-nonylphenol ethoxylates onto insoluble chitosan beads bearing cyclodextrin moieties
In order to develop a treatment method for industrial wastewater, the adsorption of 4-nonylphenol ethoxylates (NPEs), non-ionic surfactants used in the industry, onto chitosan beads having cyclodextrin (CDC be...
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Article
All-in-One Tube Method for Quantitative Gene Expression Analysis in Oligo-dT30 Immobilized PCR Tube Coated with MPC Polymer
In this report, we have developed a novel quantitative RT-PCR protocol in which the procedure including mRNA purification can be performed in an all-in-one tube. To simplify gene expression analysis, oligo-dT30 i...
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Article
Production of rosamicin derivatives in Micromonospora rosaria by introduction of d-mycinose biosynthetic gene with ΦC31-derived integration vector pSET152
Some of the polyketide-derived bioactive compounds contain sugars attached to the aglycone core, and these sugars often impart specific biological activity to the molecule or enhance this activity. Mycinamicin...
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Article
Single-Tube Genoty** from a Human Hair Root by Direct PCR
We have developed a simple, labor-saving, inexpensive and rapid SNP genoty** method that directly uses a human hair root as the template. This single-tube genoty** method was used to successfully and relia...
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Article
Genoty** of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction
We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genoty** method that works directly on whole human blood. This single-tube genoty** method was used to ...
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Article
Isolation and characterization of 23-O-mycinosyl-20-dihydro-rosamicin: a new rosamicin analogue derived from engineered Micromonospora rosaria
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Article
Production of a hybrid 16-membered macrolide antibiotic by genetic engineering of Micromonospora sp. TPMA0041
Some polyketide-derived bioactive compounds contain sugars attached to the aglycone core, and these sugars often enhance or impart specific biological activity to the molecule. Mycinamicin II, a 16-member macr...
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Article
Direct Detection of Single Nucleotide Polymorphism (SNP) by the TaqMan PCR Assay Using Dried Saliva on Water-soluble Paper and Hair-roots, without DNA Extraction
We have developed a new method for directly using unprocessed biological specimens as templates for the TaqMan assay. DNA extraction and purification had been believed to be required for the assay, but our new...
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Article
Relationships of alcohol dehydrogenase 1B (ADH1B) and aldehyde dehydrogenase 2 (ALDH2) genotypes with alcohol sensitivity, drinking behavior and problem drinking in Japanese older men
Many East Asians have the genetic polymorphisms rs1229984 in alcohol dehydrogenase 1B (ADH1B) and rs671 in aldehyde dehydrogenase 2 (ALDH2). Here we analyzed the relationships of the two genotypes with alcohol se...
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Article
Open AccessEcophysiological consequences of alcoholism on human gut microbiota: implications for ethanol-related pathogenesis of colon cancer
Chronic consumption of excess ethanol increases the risk of colorectal cancer. The pathogenesis of ethanol-related colorectal cancer (ER-CRC) is thought to be partly mediated by gut microbes. Specifically, bac...
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Article
Usefulness of transabdominal ultrasonography for assessing ulcerative colitis: a prospective, multicenter study
Transabdominal ultrasonography (US) has been reported to be a useful tool for evaluating ulcerative colitis (UC) although with less well-established data than for Crohn’s disease. This prospective multicenter ...
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Article
A Simple and Effective Method for Speciation Analysis of 13 Arsenic Species Using HPLC on a Fluorocarbon Stationary Phase Coupled to ICP-MS
The separation properties of arsenic species was investigated using HPLC-ICP-MS with several commercially available fluorocarbon stationary phases and no ion-pair reagents in HPLC. One pentafluorophenyl column...
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Article
Open AccessIntravenous tacrolimus is a superior induction therapy for acute severe ulcerative colitis compared to oral tacrolimus
Intravenous corticosteroid is the mainstay for managing acute severe ulcerative colitis, but one-third of patients do not respond to intravenous corticosteroid. Tacrolimus, a salvage therapy before colectomy, ...
