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  1. No Access

    Article

    Detection of the phosphorothioate oligonucleotide fomivirsen using a ligase detection reaction with polymerase chain reaction

    This study aimed to develop a simple and sensitive detection method for fomivirsen, a 21-nucleotide phosphorothioate oligonucleotide used as a nucleic acid medicine, using a ligase detection reaction. A ligati...

    Naoki Harikai, Haruka Kakuda, Takumi Uchiyama, Tsubaki Yamamoto… in Analytical Sciences (2024)

  2. No Access

    Article

    Application of an Alkaline and Silica Membrane DNA Extraction Method to Detect Mitochondrial DNA in Foods

    To improve the efficiency of mitochondrial DNA (mtDNA) extraction from food, a simple method, PrepM, was developed by effectively combining alkaline sodium dodecyl sulfate lysis and DNA binding to a silica mem...

    Naoki Harikai, Kazufusa Shinomiya in Food Analytical Methods (2015)

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    Article

    All-in-One Tube Method for Quantitative Gene Expression Analysis in Oligo-dT30 Immobilized PCR Tube Coated with MPC Polymer

    In this report, we have developed a novel quantitative RT-PCR protocol in which the procedure including mRNA purification can be performed in an all-in-one tube. To simplify gene expression analysis, oligo-dT30 i...

    Atsuko Tanaka, Naoki Harikai, Shin Saito, Toru Yakabe, Sohei Funaoka… in Analytical Sciences (2009)