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Article
Direct Detection of Single Nucleotide Polymorphism (SNP) by the TaqMan PCR Assay Using Dried Saliva on Water-soluble Paper and Hair-roots, without DNA Extraction
We have developed a new method for directly using unprocessed biological specimens as templates for the TaqMan assay. DNA extraction and purification had been believed to be required for the assay, but our new...
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Article
Genoty** of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction
We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genoty** method that works directly on whole human blood. This single-tube genoty** method was used to ...
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Article
Single-Tube Genoty** from a Human Hair Root by Direct PCR
We have developed a simple, labor-saving, inexpensive and rapid SNP genoty** method that directly uses a human hair root as the template. This single-tube genoty** method was used to successfully and relia...
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Article
All-in-One Tube Method for Quantitative Gene Expression Analysis in Oligo-dT30 Immobilized PCR Tube Coated with MPC Polymer
In this report, we have developed a novel quantitative RT-PCR protocol in which the procedure including mRNA purification can be performed in an all-in-one tube. To simplify gene expression analysis, oligo-dT30 i...
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Article
Conflict Among Individual Mitochondrial Proteins in Resolving the Phylogeny of Eutherian Orders
The phylogenetic relationship among primates, ferungulates (artiodactyls + cetaceans + perissodactyls + carnivores), and rodents was examined using proteins encoded by the H strand of mtDNA, with marsupials a...
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Article
Protein phylogeny of translation elongation factor EF-1α suggests microsporidians are extremely ancient eukaryotes
Partial regions of the mRNA encoding a major part of translation elongation factor 1α (EF-1α) from a mitochondrion-lacking protozoan,Glugea plecoglossi, that belongs to microsporidians, were amplified by polymera...
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Article
Evolution of the active sequences of the HpaI short interspersed elements
Ninety-nine members of the salmonid HpaI and AvaIII families of short interspersed repetitive elements (SINEs) were aligned and a general consensus sequence was deduced. The presence of 26 correlated changes in n...
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Article
Close phylogenetic relationship between vestimentifera (tube worms) and annelida revealed by the amino acid sequence of elongation factor-lα
To clarify the phylogenetic position of Vestimentifera (tube worms), 346-bp fragments of the elongation factor-lα (EF-lα) gene (939–1286 according to the numbering of the human gene) of a vestimentiferan, Lamelli...
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Evolution of Repetitive Sequences
Three families of tRNA-derived SINE (short interspersed element) in the genomes of salmonid species were isolated and characterized. These three families, the salmon Sma 1 family, charr Fok 1 family and salmon...
