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Article
SViMULATE: a computer program facilitating interactive, multi-mode simulation of analytical ultracentrifugation data
The ability to simulate sedimentation velocity (SV) analytical ultracentrifugation (AUC) experiments has proved to be a valuable tool for research planning, hypothesis testing, and pedagogy. Several options fo...
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Article
Open AccessCorrection to: Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study
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Article
Open AccessReproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study
Microscale thermophoresis (MST), and the closely related Temperature Related Intensity Change (TRIC), are synonyms for a recently developed measurement technique in the field of biophysics to quantify biomolec...
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Article
Open AccessMechanism of karyopherin-β2 binding and nuclear import of ALS variants FUS(P525L) and FUS(R495X)
Mutations in the RNA-binding protein FUS cause familial amyotropic lateral sclerosis (ALS). Several mutations that affect the proline-tyrosine nuclear localization signal (PY-NLS) of FUS cause severe juvenile ...
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Protocol
Measuring the KD of Protein–Ligand Interactions Using Microscale Thermophoresis
Microscale thermophoresis (MST) has become a widely used technique to determine the KD or EC50 of protein–ligand interactions. The method exploits the tendency of macromolecules to migrate along a thermal gradien...
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Article
Using modern approaches to sedimentation velocity to detect conformational changes in proteins
It has been known for decades that proteins undergo conformational changes in response to binding ligands. Such changes are usually accompanied by a loss of entropy by the protein, and thus conformational chan...
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Article
Open AccessMechanistic insight into TRIP13-catalyzed Mad2 structural transition and spindle checkpoint silencing
The spindle checkpoint maintains genomic stability and prevents aneuploidy. Unattached kinetochores convert the latent open conformer of the checkpoint protein Mad2 (O-Mad2) to the active closed conformer (C-M...
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Article
Open AccessStructural basis of arrestin-3 activation and signaling
A unique aspect of arrestin-3 is its ability to support both receptor-dependent and receptor-independent signaling. Here, we show that inositol hexakisphosphate (IP6) is a non-receptor activator of arrestin-3 and...
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Article
Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions
This protocol from Brautigam et al. describes methods for baseline correction and global analysis of isothermal titration calorimetry data using NITPIC and SEDPHAT. Publication-quality graphs of resulting data ca...
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Article
Structure and ligand-binding mechanism of the human OX1 and OX2 orexin receptors
Human orexin receptors (hOX1R and hOX2R) are GPCRs involved in sleep regulation. Structures of hOX1R bound to a selective antagonist or to a dual antagonist, functional assays and computational analyses reveal th...
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Article
Mechanism of actin filament nucleation by the bacterial effector VopL
VopL is a bacterial actin nucleation factor that induces actin stress fibers when injected by bacteria into eukaryotic host cells. Biochemical and structural analyses of VopL-mediated actin nucleation suggest ...
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Article
Open AccessIdentification of a bacteriocin and its cognate immunity factor expressed by Moraxella catarrhalis
Bacteriocins are antimicrobial proteins and peptides ribosomally synthesized by some bacteria which can effect both intraspecies and interspecies killing.
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Protocol
Methods for Analysis of Ligand-Induced RNA Conformational Changes
Encoded within many RNA sequences is the requisite information for folding of intricate three-dimensional structures. Moreover, many noncoding RNAs can adopt structurally distinct and functionally specialized ...
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Article
Structural basis of histone demethylation by LSD1 revealed by suicide inactivation
Histone methylation regulates diverse chromatin-templated processes, including transcription. The recent discovery of the first histone lysine–specific demethylase (LSD1) has changed the long-held view that hi...
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Article
Side chain and backbone contributions of Phe508 to CFTR folding
Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), an integral membrane protein, cause cystic fibrosis (CF). The most common CF-causing mutant, deletion of Phe508, fails to properly f...
