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Article
Open AccessImpact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation
Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen that forms robust biofilms in the different niches it occupies. Numerous physiological adaptations are required as this organism shifts from soil or a...
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Article
Open AccessSelective pressures during chronic infection drive microbial competition and cooperation
Chronic infections often contain complex mixtures of pathogenic and commensal microorganisms ranging from aerobic and anaerobic bacteria to fungi and viruses. The microbial communities present in infected tiss...
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Article
Open AccessThe innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction
Microorganisms form biofilms containing differentiated cell populations. To determine factors driving differentiation, we herein visualize protein and metal distributions within Pseudomonas aeruginosa biofilms us...
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Protocol
Methods for Analysis of Ligand-Induced RNA Conformational Changes
Encoded within many RNA sequences is the requisite information for folding of intricate three-dimensional structures. Moreover, many noncoding RNAs can adopt structurally distinct and functionally specialized ...
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Protocol
Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2’-Hydroxyl Acylation and Primer Extension
RNA sequences fold upon themselves to form complex structures. Functional analysis of most biological RNAs requires knowledge of secondary structure arrangements and tertiary base interactions. Therefore, rapi...
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Protocol
Structural Probing Techniques on Natural Aptamers
RNA sequences fold in a hierarchical manner to form complex structures. This folding pathway proceeds first with formation of secondary structure elements followed by the compilation of tertiary contacts. Alth...