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Article
Cas1 mediates the interference stage in a phage-encoded CRISPR–Cas system
Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas systems are prokaryotic adaptive immune systems against invading phages and other mobile genetic elements. Notably, some phages, including...
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Article
Structural optimization, interfacial contact, and transverse thermoelectric properties of Ag2Te/Bi0.5Sb1.5Te3 artificially tilted multilayer thermoelectric devices
Artificially tilted multilayer thermoelectric devices (ATMTDs) have attracted widespread attention due to their advantages of simple and stable structure. In this study, n-type Ag2Te and p-type Bi0.5Sb1.5Te3 (BiS...
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Article
Open AccessMolecular basis for the catalytic mechanism of human neutral sphingomyelinases 1 (hSMPD2)
Enzymatic breakdown of sphingomyelin by sphingomyelinase (SMase) is the main source of the membrane lipids, ceramides, which are involved in many cellular physiological processes. However, the full-length stru...
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Article
Open AccessA conformation-locking inhibitor of SLC15A4 with TASL proteostatic anti-inflammatory activity
Dysregulation of pathogen-recognition pathways of the innate immune system is associated with multiple autoimmune disorders. Due to the intricacies of the molecular network involved, the identification of path...
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Article
Open AccessStructural basis for recruitment of TASL by SLC15A4 in human endolysosomal TLR signaling
Toll-like receptors (TLRs) are a class of proteins that play critical roles in recognizing pathogens and initiating innate immune responses. TASL, a recently identified innate immune adaptor protein for endoly...
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Article
Molecular basis of the plant ROS1-mediated active DNA demethylation
Active DNA demethylation plays a crucial role in eukaryotic gene imprinting and antagonizing DNA methylation. The plant-specific REPRESSOR OF SILENCING 1/DEMETER (ROS1/DME) family of enzymes directly excise 5-...
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Article
Open AccessStructural basis of a two-antibody cocktail exhibiting highly potent and broadly neutralizing activities against SARS-CoV-2 variants including diverse Omicron sublineages
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs), especially the latest Omicron, have exhibited severe antibody evasion. Broadly neutralizing antibodies with high pot...
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Article
Discovery of small-molecule activators of nicotinamide phosphoribosyltransferase (NAMPT) and their preclinical neuroprotective activity
The decline of nicotinamide adenine dinucleotide (NAD) occurs in a variety of human pathologies including neurodegeneration. NAD-boosting agents can provide neuroprotective benefits. Here, we report the discov...
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Article
AcrIF5 specifically targets DNA-bound CRISPR-Cas surveillance complex for inhibition
CRISPR-Cas systems are prokaryotic antiviral systems, and phages use anti-CRISPR proteins (Acrs) to inactivate these systems. Here we present structural and functional analyses of AcrIF5, exploring its unique ...
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Article
Open AccessInsights into the inhibition of type I-F CRISPR-Cas system by a multifunctional anti-CRISPR protein AcrIF24
CRISPR-Cas systems are prokaryotic adaptive immune systems and phages use anti-CRISPR proteins (Acrs) to counteract these systems. Here, we report the structures of AcrIF24 and its complex with the crRNA-guide...
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Article
The coupling mechanism of mammalian mitochondrial complex I
Mammalian respiratory complex I (CI) is a 45-subunit, redox-driven proton pump that generates an electrochemical gradient across the mitochondrial inner membrane to power ATP synthesis in mitochondria. In the ...
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Article
Open AccessCryo-EM structures and transport mechanism of human P5B type ATPase ATP13A2
Polyamines are important polycations that play critical roles in mammalian cells. ATP13A2 belongs to the orphan P5B adenosine triphosphatases (ATPase) family and has been established as a lysosomal polyamine e...
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Article
Open AccessMolecular insights into the human ABCB6 transporter
ABCB6 plays a crucial role in energy-dependent porphyrin transport, drug resistance, toxic metal resistance, porphyrin biosynthesis, protection against stress, and encoding a blood group system Langereis antig...
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Article
Open AccessStructure of the full-length human Pannexin1 channel and insights into its role in pyroptosis
Pannexin1 (PANX1) is a large-pore ATP efflux channel with a broad distribution, which allows the exchange of molecules and ions smaller than 1 kDa between the cytoplasm and extracellular space. In this study, ...
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Article
Open AccessStructure of intact human MCU supercomplex with the auxiliary MICU subunits
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Open AccessThe structural basis of function and regulation of neuronal cotransporters NKCC1 and KCC2
NKCC and KCC transporters mediate coupled transport of Na++K++Cl− and K++Cl− across the plasma membrane, thus regulating cell Cl− concentration and cell volume and playing critical roles in transepithelial salt a...
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Article
Open AccessAtomic structure of human TOM core complex
The translocase of the outer mitochondrial membrane (TOM) complex is the main entry gate for mitochondrial precursor proteins synthesized on cytosolic ribosomes. Here we report the single-particle cryo-electro...
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Article
Open AccessResearch journey of respirasome
Respirasome, as a vital part of the oxidative phosphorylation system, undertakes the task of transferring electrons from the electron donors to oxygen and produces a proton concentration gradient across the in...
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Article
Distinct structural modulation of photosystem I and lipid environment stabilizes its tetrameric assembly
Photosystem I (PSI) is able to form different oligomeric states across various species. To reveal the structural basis for PSI dimerization and tetramerization, we structurally investigated PSI from the cyanob...
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Article
Open AccessCorrection to: A binding-block ion selective mechanism revealed by a Na/K selective channel
In the original publication the PDB numbers were not cited.
