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Article
Open AccessThe intrinsic substrate specificity of the human tyrosine kinome
Phosphorylation of proteins on tyrosine (Tyr) residues evolved in metazoan organisms as a mechanism of coordinating tissue growth1. Multicellular eukaryotes typically have more than 50 distinct protein Tyr kinase...
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Article
A multi-laboratory benchmark study of isothermal titration calorimetry (ITC) using Ca2+ and Mg2+ binding to EDTA
A small-scale ITC benchmarking study was performed involving 9 biophysics laboratories/facilities, to evaluate inter-laboratory and intra-laboratory basal levels of uncertainty. Our prime goal was to assess a ...
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Protocol
Measuring the KD of Protein–Ligand Interactions Using Microscale Thermophoresis
Microscale thermophoresis (MST) has become a widely used technique to determine the KD or EC50 of protein–ligand interactions. The method exploits the tendency of macromolecules to migrate along a thermal gradien...
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Article
Using modern approaches to sedimentation velocity to detect conformational changes in proteins
It has been known for decades that proteins undergo conformational changes in response to binding ligands. Such changes are usually accompanied by a loss of entropy by the protein, and thus conformational chan...
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Article
The Role of the Supernumerary Subunit of Rhodobactersphaeroides Cytochrome bc 1 Complex
The smallest molecular weight subunit (subunit IV), which contains no redox prosthetic group,is the only supernumerary subunit in the four-subunit Rhodobacter sphaeroides bc 1 complex.This subunit is involved in ...
