13 Result(s)
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Life Sciences
Biomedical Engineering/Biotechnology
Plant Genetics and Genomics
Transgenics
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Article
Improved production of GTKO/hCD55/hCD59 triple-gene-modified Diannan miniature pigs for xenotransplantation by recloning
Multiple genetic modification is necessary for successful xenotransplantation from pigs. However, multiple-genetically modified cells usually suffer from various drug selections and long-term in vitro culture,...
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Insights into the regulatory characteristics of silkworm fibroin gene promoters using a modified Gal4/UAS system
The silkworm Bombyx mori is a valuable insect that synthesizes bulk amounts of fibroin protein in its posterior silk gland (PSG) and weaves these proteins into silk cocoons. The mechanism by which the fibroin pro...
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Article
Expression of the high molecular weight glutenin 1Ay gene from Triticum urartu in barley
In this study, we successfully expressed the active 1Ay subunit of Triticum urartu in barley by Agrobacterium-mediated transformation with a transformation efficiency of 19.9%. The results of SDS–PAGE revealed th...
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Article
Highly efficient and inducible DNA excision in transgenic silkworms using the FLP/FRT site-specific recombination system
Efficient and inducible recombinase-mediated DNA excision is an optimal technology for automatically deleting unwanted DNA sequences, including selection marker genes. However, this methodology has yet to be e...
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Article
The aflatoxin-detoxifizyme specific expression in mouse parotid gland
The aflatoxin-detoxifizyme (ADTZ) gene derived from Armillariella tabescens was cloned into parotid gland-specific expression vector (pPSPBGPneo) to construct the parotid gland-specific vector expressing ADTZ (pP...
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Article
Open AccessGenome edited sheep and cattle
Genome editing tools enable efficient and accurate genome manipulation. An enhanced ability to modify the genomes of livestock species could be utilized to improve disease resistance, productivity or breeding ...
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Article
OsLOX2, a rice type I lipoxygenase, confers opposite effects on seed germination and longevity
Rice production and seed storage are confronted with grain deterioration and loss of seed viability. Some members of the lipoxygenase (LOX) family function in degradation of storage lipids during the seed germ...
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Article
Eliminating host-mediated effects demonstrates Bt maize producing Cry1F has no adverse effects on the parasitoid Cotesia marginiventris
The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is an important pest of maize in the United States and many tropical areas in the western hemisphere. In 2001, Herculex I® (Cry1F) ...
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Article
The high-level accumulation of n-3 polyunsaturated fatty acids in transgenic pigs harboring the n-3 fatty acid desaturase gene from Caenorhabditis briggsae
Livestock meat is generally low in n-3 polyunsaturated fatty acids (PUFAs), which are beneficial to human health. An alternative approach to increasing the levels of n-3 PUFAs in meat is to generate transgenic...
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Article
Gene transfer and mutagenesis mediated by Slee** Beauty transposon in Nile tilapia (Oreochromis niloticus)
The success of gene transfer has been demonstrated in many of vertebrate species, whereas the efficiency of producing transgenic animals remains pretty low due to the random integration of foreign genes into a...
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Article
Transgenic sheep generated by lentiviral vectors: safety and integration analysis of surrogates and their offspring
The safety of HIV-1 based vectors was evaluated during the production of transgenic sheep. Vectors were introduced into the perivitelline space of in vivo derived one-cell sheep embryos by microinjection then ...
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Article
Genetic engineering of a Lemna isoleucine auxotroph
Lemna, a member of the Lemnaceae or duckweed family, is a small aquatic plant that can be quickly transformed to produce recombinant proteins in a contained and controlled bioprocessing environme...
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Article
Transgenic Tobacco and Apple Plants Expressing Biotin-binding Proteins are Resistant to two Cosmopolitan Insect Pests, Potato Tuber Moth and Lightbrown Apple Moth, Respectively
Tobacco (Nicotiana tabacum cv. Samsun) and apple (Malus × domestica cv. Royal Gala) plants expressing avidin or strepavidin were produced using Agrobacterium tumefaciens-mediated transformation. ELISA assays show...
