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Article
DFS-KeyLevel: A Two-Layer Test Scenario Generation Approach for UML Activity Diagram
For automatic generation of test scenarios from UML (Unified Modeling Language) activity diagrams (ADs) are very important for improving test efficiency. However, state-of-the-art approaches mainly focus on si...
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Article
Open AccessClinical nurses’ moral courage and related factors: an empowerment perspective
Moral courage as part of the moral competence of nurses has received increasing attention. Determination of the factors affecting moral courage is important in improving the quality of care. The purpose of thi...
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Article
Open AccessPreliminary evaluation of the Chinese version of the patient-reported outcomes measurement information system 29-item profile in patients with aortic dissection
The Patient-Reported Outcomes Measurement Information System 29-item Profile (PROMIS-29) has been widely used to measure health outcomes from the patient’s perspective. It has not been validated in adults with...
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Article
The development of transgenic mice for the expression of large amounts of human lysozyme in milk
Human lysozyme (hLYZ) has important potential applications as antimicrobial medicine and food additive. To develop a robust expression vector that ensures expression of large amounts of hLYZ in milk, here a 26...
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Article
The high-level accumulation of n-3 polyunsaturated fatty acids in transgenic pigs harboring the n-3 fatty acid desaturase gene from Caenorhabditis briggsae
Livestock meat is generally low in n-3 polyunsaturated fatty acids (PUFAs), which are beneficial to human health. An alternative approach to increasing the levels of n-3 PUFAs in meat is to generate transgenic...
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Article
The extremely high level expression of human serum albumin in the milk of transgenic mice
The recombinant production of human serum albumin has been challenging due to the low unit price and huge amount needed, for the commercial production of rhSA at an economically feasible level, It will be well...
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Article
The High-Level Expression of Human Tissue Plasminogen Activator in the Milk of Transgenic Mice with Hybrid Gene Locus Strategy
Transgene expression for the mammary gland bioreactor aimed at producing recombinant proteins requires optimized expression vector construction. Previously we presented a hybrid gene locus strategy, which was ...
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Article
Plk1-mediated phosphorylation of UAP56 regulates the stability of UAP56
Polo-like kinase 1 (Plk1) is a conserved serine/threonine protein kinase that plays pivotal roles during the cell cycle and cell proliferation. Although a number of important targets have been identified, the ...
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Article
Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer
Omega-3(ω-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of ω-3 fatty acids in the...
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Article
A mWAP–hLF hybrid gene locus gave extremely high level expression of human lactoferrin in the milk of transgenic mice
The production of pharmaceuticals by current mammary gland bioreactor techniques is limited by the low expression level of foreign proteins. We describe here a novel method that solves this problem. A successi...
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Article
A modified n–3 fatty acid desaturase gene from Caenorhabditis briggsae produced high proportion of DHA and DPA in transgenic mice
The functions of polyunsaturated fatty acids (PUFAs) have been widely investigated. In mammals, levels of n−3 PUFAs are relatively low compared to those of n−6 PUFAs. Either a lack of n−3 PUFAs or an excess of n−...
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Article
Measurement of Indoor Sulfur Dioxide Emission from Coal–Biomass Briquettes
The coal–biomass briquette is an alternative fuel that replaces low-grade coal. It is expected that the use of coal–biomass briquettes will effectively reduce the indoor concentration of sulfur dioxide (SO2) emit...
