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  1. No Access

    Article

    Expression of various biotin-binding proteins in transgenic tobacco confers resistance to potato tuber moth, Phthorimaea operculella (Zeller) (fam. Gelechiidae)

    The high affinity biotin-binding proteins (BBPs) avidin and streptavidin are established insecticidal agents, effective against a range of insect pests. Earlier work showed that, when expressed in planta, full...

    Colleen Murray, Ngaire P. Markwick, Ryohei Kaji, Joanne Poulton… in Transgenic Research (2010)

  2. No Access

    Article

    Potato Transformation with Modified Nucleotide Sequences of the cry9Aa2 Gene Improves Resistance to Potato Tuber Moth

    The efficacy of four versions of a cry9Aa2 gene was evaluated against larvae of potato tuber moth (PTM; Phthorimaea operculella Zeller) following transfer to potato (Solanum tuberosum L.). A truncated native cry9...

    Jeanne M. E. Jacobs, Michael F. G. Takla, Lisa C. Docherty… in Potato Research (2009)

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    Article

    Expression of two heterologous proteins depends on the mode of expression: comparison of in vivo and in vitro methods

    The yield of two proteins, avidin and green fluorescent protein (GFP), expressed from a modified Autographa californica nucleopolyhedrovirus (AcMNPV), was compared in Sf9 cell culture monolayer, Sf21 cell suspens...

    Laurence N. Gatehouse, Ngaire P. Markwick… in Bioprocess and Biosystems Engineering (2008)

  4. No Access

    Article

    Transgenic Tobacco and Apple Plants Expressing Biotin-binding Proteins are Resistant to two Cosmopolitan Insect Pests, Potato Tuber Moth and Lightbrown Apple Moth, Respectively

    Tobacco (Nicotiana tabacum cv. Samsun) and apple (Malus × domestica cv. Royal Gala) plants expressing avidin or strepavidin were produced using Agrobacterium tumefaciens-mediated transformation. ELISA assays show...

    Ngaire P. Markwick, Lisa C. Docherty, Margaret M. Phung… in Transgenic Research (2003)

  5. No Access

    Article

    The Expression of a Mammalian Proteinase Inhibitor, Bovine Spleen Trypsin Inhibitor in Tobacco and its Effects on Helicoverpa armigera Larvae

    The cDNA for bovine spleen trypsin inhibitor (SI), a homologue of bovine pancreatic trypsin inhibitor (BPTI), including the natural mammalian presequence was expressed in tobacco using Agrobacterium tumefaciens-m...

    John T. Christeller, Elisabeth P.J. Burgess, Valentina Mett… in Transgenic Research (2002)

  6. No Access

    Article

    Enhanced expression of the shape Bacillus thuringiensis cry9Aa2 gene in transgenic plants by nucleotide sequence modification confers resistance to potato tuber moth

    The Bacillus thuringiensis cry9Aa2 gene encodes a 129 kDa protein with insecticidal activity against Lepidoptera, including the larvae of potato tuber moth (Phthorimaea operculella). The insecticidal moiety of...

    Andrew P. Gleave, Deepali S. Mitra, Ngaire P. Markwick… in Molecular Breeding (1998)

  7. No Access

    Article

    The avoidance of superparasitism in four species of parasitic wasp—Mathematical models and experimental results

    Experimental results, and interpretation, are presented which relate to oviposition behaviour in four species of parasitic wasp (Hymenoptera: Chalcidoidea). All lay their eggs in pupae of the common house fly (Mu...

    J. H. Maindonald, Ngaire P. Markwick in Researches on Population Ecology (1986)