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Article
Open AccessMendelian gene identification through mouse embryo viability screening
The diagnostic rate of Mendelian disorders in sequencing studies continues to increase, along with the pace of novel disease gene discovery. However, variant interpretation in novel genes not currently associa...
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Article
A transcriptome atlas of silkworm silk glands revealed by PacBio single-molecule long-read sequencing
The silk gland of the silkworm Bombyx mori is a specialized organ where silk proteins are efficiently synthesized under precise regulation that largely determines the properties of silk fibers. To understand the ...
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Article
Open AccessAnnular flexible thermoelectric devices with integrated-module architecture
Organic and composite thermoelectric (TE) materials have witnessed explosive developments in recent years. Design strategy of their flexible devices is vital to achieve high performance and suit various applic...
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Article
Insights into the regulatory characteristics of silkworm fibroin gene promoters using a modified Gal4/UAS system
The silkworm Bombyx mori is a valuable insect that synthesizes bulk amounts of fibroin protein in its posterior silk gland (PSG) and weaves these proteins into silk cocoons. The mechanism by which the fibroin pro...
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Article
Expression and characterization of recombinant human VEGF165 in the middle silk gland of transgenic silkworms
Recombinant human vascular endothelial growth factor (rhVEGF) has important applications in therapeutic angiogenesis and inhibition of VEGF-mediated pathological angiogenesis. Previous studies have shown that ...
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Article
Open AccessActivated NK cells cause placental dysfunction and miscarriages in fetal alloimmune thrombocytopenia
Miscarriage and intrauterine growth restriction (IUGR) are devastating complications in fetal/neonatal alloimmune thrombocytopenia (FNAIT). We previously reported the mechanisms for bleeding diatheses, but it ...
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Article
Open AccessIn vivo quantification of embryonic and placental growth during gestation in mice using micro-ultrasound
Non-invasive micro-ultrasound was evaluated as a method to quantify intrauterine growth phenotypes in mice. Improved methods are required to accelerate research using genetically-altered mice to investigate th...
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Article
Open AccessHigh resolution ultrasound-guided microinjection for interventional studies of early embryonic and placental development in vivoin mice
In utero microinjection has proven valuable for exploring the developmental consequences of altering gene expression, and for studying cell lineage or migration during the latter half of embryonic mouse develo...
