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The aflatoxin-detoxifizyme specific expression in mouse parotid gland

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Abstract

The aflatoxin-detoxifizyme (ADTZ) gene derived from Armillariella tabescens was cloned into parotid gland-specific expression vector (pPSPBGPneo) to construct the parotid gland-specific vector expressing ADTZ (pPSPBGPneo-ADTZ). Transgenic mice were generated by microinjection and identified by using PCR and Southern blotting analysis. PCR and Southern blotting analysis showed that total six transgenic mice carried the ADTZ gene were generated. RT-PCR analysis indicated that the expression of ADTZ mRNA could be detected only in parotid glands of the transgenic mice. The ADTZ activity in the saliva was found to be 3.72 ± 1.64 U/mL. After feeding a diet containing aflatoxin B1 (AFB1) for 14 days, the effect of ADTZ on serum biochemical indexes and AFB1 residues in serum and liver of mice were evaluated. The results showed that total protein and globulin contents in the test treatment (transgenic mice) produced ADTZ were significantly higher than that of the positive control, while alanine aminotransferase and aspartate aminotransferase activity in serum of the test treatment (transgenic mice) were remarkably lower compared to that of the positive control (P < 0.05). Moreover, AFB1 residues in serum and liver of the test treatment (transgenic mice) were significantly lower compared with that of the positive control (P < 0.05). These results in the study confirmed that ADTZ produced in transgenic mice could reduce, even eliminate the negative effects of AFB1 on mice.

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Abbreviations

PSP:

Parotid secretory protein promoter

AFB1:

Aflatoxin B1

ADTZ:

Aflatoxin-detoxifizyme

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Acknowledgments

We thank Prof. Zhang Yong-liang of South China Agriculture University for providing plasmid pPSPBGPneo. This work was also supported by Doctoral Foundation of Yanbian University.

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    Authors and Affiliations

    1. Agriculture College, Yanbian University, Gongyuan Road, Yanji, 133000, China

      Li-zeng Guan, **-shun Cai & Long-zheng Yu

    2. Institute of Animal Science, Guangdong Academy of Agriculture Science, Guangzhou, 510640, China

      Yu-** Sun

    3. Liaoning Medical University, **zhou, 121001, China

      Han-dong Wu

    4. College of Animal Science, SCAU-Alltech Research Joint Alliance, South China Agricultural University, Guangzhou, 510642, China

      Yong-liang Zhang & Qian-yun **

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    Correspondence to Li-zeng Guan.

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    Li-zeng Guan and Yu-** Sun have contributed equally to this work.

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    Guan, Lz., Sun, Yp., Cai, Js. et al. The aflatoxin-detoxifizyme specific expression in mouse parotid gland. Transgenic Res 24, 489–496 (2015). https://doi.org/10.1007/s11248-015-9863-y

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    • DOI: https://doi.org/10.1007/s11248-015-9863-y

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