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Article
Insights into the regulatory characteristics of silkworm fibroin gene promoters using a modified Gal4/UAS system
The silkworm Bombyx mori is a valuable insect that synthesizes bulk amounts of fibroin protein in its posterior silk gland (PSG) and weaves these proteins into silk cocoons. The mechanism by which the fibroin pro...
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Article
Highly efficient and inducible DNA excision in transgenic silkworms using the FLP/FRT site-specific recombination system
Efficient and inducible recombinase-mediated DNA excision is an optimal technology for automatically deleting unwanted DNA sequences, including selection marker genes. However, this methodology has yet to be e...
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Article
Open AccessAn efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori
We developed an efficient strategy that combines a method for the post-integration elimination of all transposon sequences, a site-specific recombination system and an optimized fibroin H-chain expression syst...
