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Article
Carbon export from seaweed forests to deep ocean sinks
The coastal ocean represents an important global carbon sink and is a focus for interventions to mitigate climate change and meet the Paris Agreement targets while supporting biodiversity and other ecosystem f...
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Protocol
Targeted Gene Editing in Porcine Germ Cells
As the genetic mutations driving human disease are identified, there is an increasing need for a biomedical model that can accurately represent the disease of interest and provide a platform for potential ther...
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Article
Author Correction: Dysregulated ribonucleoprotein granules promote cardiomyopathy in RBM20 gene-edited pigs
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Article
Open AccessThe NIH Somatic Cell Genome Editing program
The move from reading to writing the human genome offers new opportunities to improve human health. The United States National Institutes of Health (NIH) Somatic Cell Genome Editing (SCGE) Consortium aims to a...
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Article
Dysregulated ribonucleoprotein granules promote cardiomyopathy in RBM20 gene-edited pigs
Ribonucleoprotein (RNP) granules are biomolecular condensates—liquid–liquid phase-separated droplets that organize and manage messenger RNA metabolism, cell signaling, biopolymer assembly, biochemical reaction...
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Article
A reduction of primary cilia but not hedgehog signaling disrupts morphogenesis in testicular organoids
Most mammalian cells possess a single, non-motile primary cilium that plays an important role in mediating cellular signaling pathways, such as Hedgehog (Hh) signaling. Primary cilia are present on testicular ...
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Article
Open AccessGenetically engineered minipigs model the major clinical features of human neurofibromatosis type 1
Neurofibromatosis Type 1 (NF1) is a genetic disease caused by mutations in Neurofibromin 1 (NF1). NF1 patients present with a variety of clinical manifestations and are predisposed to cancer development. Many NF1...
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Article
Production of hornless dairy cattle from genome-edited cell lines
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Article
Open AccessEfficient introgression of allelic variants by embryo-mediated editing of the bovine genome
The recent development of designer nucleases allows for the efficient and precise introduction of genetic change into livestock genomes. Most studies so far have focused on the introduction of random mutations...
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Article
Open AccessGenome edited sheep and cattle
Genome editing tools enable efficient and accurate genome manipulation. An enhanced ability to modify the genomes of livestock species could be utilized to improve disease resistance, productivity or breeding ...
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Article
Open AccessLive pigs produced from genome edited zygotes
Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both...
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Article
In vivo genome editing using a high-efficiency TALEN system
Although zebrafish is an important animal model for basic vertebrate biology and human disease modelling, rapid targeted genome modification has not been possible in this species; here a technique based on imp...
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Article
Strategies for selection marker-free swine transgenesis using the Slee** Beauty transposon system
Swine transgenesis by pronuclear injection or cloning has traditionally relied on illegitimate recombination of DNA into the pig genome. This often results in animals containing concatemeric arrays of transgen...
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Article
Efficient mammalian germline transgenesis by cis-enhanced Slee** Beauty transposition
Heightened interest in relevant models for human disease increases the need for improved methods for germline transgenesis. We describe a significant improvement in the creation of transgenic laboratory mice a...
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Article
Deducing an upper bound to the horizontal eddy diffusivity using a stochastic Lagrangian model
We present a method for estimating the upper bound of the horizontal eddy diffusivity using a non-stationary Lagrangian stochastic model. First, we identify a mixing barrier using a priori evidence (e.g., aeri...
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Article
Pigs taking wing with transposons and recombinases
Swine production has been an important part of our lives since the late Mesolithic or early Neolithic periods, and ranks number one in world meat production. Pig production also contributes to high-value-added...
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Article
Open AccessEnzymatic engineering of the porcine genome with transposons and recombinases
Swine is an important agricultural commodity and biomedical model. Manipulation of the pig genome provides opportunity to improve production efficiency, enhance disease resistance, and add value to swine produ...
