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Article
Two antigen-independent adhesion pathways used by human cytotoxic T-cell clones
Cell–cell adhesion is essential for many immunological functions1–4, including interaction of cytotoxic T lymphocytes (CTLs) with their targets5–8. We have explored CTL-target interactions using well-characterize...
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Article
No T-cell tyrosine protein kinase signalling or calcium mobilization after CD4 association with HIV-1 or HIV-1 gp120
THE T lymphocyte surface protein CD4 is an integral membrane glycoprotein noncovalently associated with the tyrosine protein kinase p56lck (reviewed in ref. 1). In normal T cells, surface association of CD4 molec...
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Article
IMPAIRED IMMUNE RECONSTITUTION POST SEQUENTIAL HIGH-DOSE CHEMOTHERAPY AND PERIPHERAL BLOOD PROGENITOR CELL (PBPC) INFUSION. † 962
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Article
SKEWING OF THE T CELL REPERTOIRE TOWARD A TARGET ANTIGEN DURING A PERIOD OF T CELL IMMUNE RECONSTITUTION. • 935
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Chapter
Langerhans Cells in the TGFβ1 Null Mouse
We recently identified a cell surface protein (gp40) that is homologous to human EpCAM, a putative homophilic adhesion molecule, and that is abundantly expressed by some murine dendritic cells (including Lang...
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Protocol
Thymic Involution
The thymus contributes to the regulation of tolerance and the prevention of autoimmunity at many levels. First, auto-reactive CD4+ and CD8+ T cells are clonally deleted during negative selection in the thymus, es...
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Chapter and Conference Paper
Interleukin-7 Immunotherapy
IL-7 is a member of the common γ -chain family of cytokines sharing a common γ -chain in their receptor. Beyond its long-established pivotal role in immune development, it has been more recently recognized as ...
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Chapter
Immune Reconstitution and Implications for Immunotherapy Following Hematopoeitic Stem Cell Transplantation
The innate immune system comprises a collection of cells that recognize and eradicate pathogens or aberrant cells without priming or antigen presentation. Natural killer cells, neutrophils, monocytes, dendriti...
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Article
Signaling by intrathymic cytokines, not T cell antigen receptors, specifies CD8 lineage choice and promotes the differentiation of cytotoxic-lineage T cells
Whether CD8-lineage specification and CD8+ T cell differentiation is dependent on intrathymic cytokines signaling is unclear. Singer and co-workers show that interleukin 7 specifies CD8-lineage choice and promote...
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Article
Harnessing the biology of IL-7 for therapeutic application
Interleukin-7 (IL-7) is required for T cell development in mice and humans and is produced by stromal tissues rather than activated lymphocytes. Under normal c...
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Protocol
Bone Marrow and Fetal Liver Radiation Chimeras
Radiation chimeras are prepared by subjecting recipient mice to sublethal or lethal dose of irradiation and injecting them with hematopoietic stem cells (HSC) from untreated donor mice. HSC can be obtained fro...
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Protocol
Analysis of Cell Proliferation and Homeostasis Using EdU Labeling
Determination of cellular proliferation and population turnover is an important tool for research on lymphoid cell function. Historically this has been done using radiolabeled nucleotides or nucleoside analogs...
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Article
Open AccessComparing DNA enrichment of proliferating cells following administration of different stable isotopes of heavy water
Deuterated water (2H2O) is a label commonly used for safe quantitative measurement of deuterium enrichment into DNA of proliferating cells. More recently, it has been used for labeling proteins and other biomolec...
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Article
Impact of the 2014 NIH chronic graft-versus-host disease scoring criteria modifications assessed in a large cohort of severely affected patients
In 2005, the National Institutes of Health (NIH) chronic graft-versus-host disease (cGVHD) consensus project provided diagnosis and staging criteria, based mostly on clinical experience and expert opinion. The...
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Article
Safety and feasibility of anti-CD19 CAR T cells with fully human binding domains in patients with B-cell lymphoma
Anti-CD19 chimeric antigen receptor (CAR)-expressing T cells are an effective treatment for B-cell lymphoma, but often cause neurologic toxicity. We treated 20 patients with B-c...
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Article
Author Correction: Safety and feasibility of anti-CD19 CAR T cells with fully human binding domains in patients with B-cell lymphoma
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Siglec-6 is a target for chimeric antigen receptor T-cell treatment of chronic lymphocytic leukemia
The only current curative treatment for chronic lymphocytic leukemia (CLL) is allogenic hematopoietic stem cell transplantation. Chimeric antigen receptor treatment targeting CD19 for CLL achieved some complet...
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Article
Open AccessIn vivo deuterium magnetic resonance imaging of xenografted tumors following systemic administration of deuterated water
In vivo deuterated water (2H2O) labeling leads to deuterium (2H) incorporation into biomolecules of proliferating cells and provides the basis for its use in cell kinetics research. We hypothesized that rapidly p...
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Article
Microbial ligand-independent regulation of lymphopoiesis by NOD1
Aberrant differentiation of progenitor cells in the hematopoietic system is known to severely impact host immune responsiveness. Here we demonstrate that NOD1, a cytosolic innate sensor of bacterial peptidogly...
