Abstract
Radiation chimeras are prepared by subjecting recipient mice to sublethal or lethal dose of irradiation and injecting them with hematopoietic stem cells (HSC) from untreated donor mice. HSC can be obtained from bone marrow or fetal liver. This technique is a powerful tool when coupled with gene targeting strategies to investigate function of HSCs, thymocyte development, and T cell function. This protocol describes how to produce bone marrow or fetal liver chimeras.
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Acknowledgement
The Intramural Research Program of the National Cancer Institute at the National Institutes of Health supports the authors. The authors have no conflicts of interest to disclose.
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Flomerfelt, F.A., Gress, R.E. (2016). Bone Marrow and Fetal Liver Radiation Chimeras. In: Bosselut, R., S. Vacchio, M. (eds) T-Cell Development. Methods in Molecular Biology, vol 1323. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2809-5_9
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DOI: https://doi.org/10.1007/978-1-4939-2809-5_9
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2808-8
Online ISBN: 978-1-4939-2809-5
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