Abstract
Determination of cellular proliferation and population turnover is an important tool for research on lymphoid cell function. Historically this has been done using radiolabeled nucleotides or nucleoside analogs, such as BrdU (5-bromo-2-deoxyuridine), that are incorporated into nascent DNA during S-phase. Recently, a new procedure was developed to label nascent DNA using EdU (5-Ethynyl-2-deoxyuridine). This new method overcomes limitations imposed by the procedure used to detect BrdU because EdU detection is based on an easily performed chemical reaction that does not require DNA denaturation, is quick and reproducible, and has a superior signal-to-noise ratio. This technique offers a wide range of opportunities to analyze cellular proliferation, population homeostasis, and cell marking procedures.
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Acknowledgement
The Intramural Research Program of the National Cancer Institute at the National Institutes of Health supports the authors. The authors thank Kevin Chua for comments on the paper. The authors have no conflicts of interest to disclose.
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Flomerfelt, F.A., Gress, R.E. (2016). Analysis of Cell Proliferation and Homeostasis Using EdU Labeling. In: Bosselut, R., S. Vacchio, M. (eds) T-Cell Development. Methods in Molecular Biology, vol 1323. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2809-5_18
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DOI: https://doi.org/10.1007/978-1-4939-2809-5_18
Publisher Name: Humana Press, New York, NY
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