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Article
Open AccessNovel principles of gamma-retroviral insertional transcription activation in murine leukemia virus-induced end-stage tumors
Insertional mutagenesis screens of retrovirus-induced mouse tumors have proven valuable in human cancer research and for understanding adverse effects of retroviral-based gene therapies. In previous studies, t...
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Article
Open AccessAn autoimmune disease prevented by anti-retroviral drugs
Both Aicardi-Goutières syndrome, a Mendelian mimic of congenital infection, and the autoimmune disease systemic lupus erythematosus can result from mutations in the gene encoding the enzyme Trex1. In mice, the...
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Article
Open AccessA retroviral mutagenesis screen identifies Cd74 as a common insertion site in murine B-lymphomas and reveals the existence of a novel IFNγ-inducible Cd74 isoform
Insertional mutagenesis screens in the mouse are an acknowledged approach to identify genes involved in the pathogenesis of cancer. The potential of these screens to identify genes causally involved in tumorig...
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Article
Open AccessOrphan receptor GPR110, an oncogene overexpressed in lung and prostate cancer
GPR110 is an orphan G protein-coupled receptor--a receptor without a known ligand, a known signaling pathway, or a known function. Despite the lack of information, one can assume that orphan receptors have imp...
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Article
Open AccessIdentification of novel Bach2 transcripts and protein isoforms through tagging analysis of retroviral integrations in B-cell lymphomas
The Bach2 gene functions as a transcriptional repressor in B-cells, showing high expression level only before the plasma cell stage. Several lines of evidence indicate that Bach2 is a B-cell specific tumor suppre...
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Article
Open AccessPvt1-encoded microRNAs in oncogenesis
The functional significance of the Pvt1 locus in the oncogenesis of Burkitt's lymphoma and plasmacytomas has remained a puzzle. In these tumors, Pvt1 is the site of reciprocal translocations to immunoglobulin loc...
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Article
Open AccessImpairment of alternative splice sites defining a novel gammaretroviral exon within gagmodifies the oncogenic properties of Akv murine leukemia virus
Mutations of an alternative splice donor site located within the gag region has previously been shown to broaden the pathogenic potential of the T-lymphomagenic gammaretrovirus Moloney murine leukemia virus, whil...
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Article
Open AccessRetroviral activation of the mir-106a microRNA cistron in T lymphoma
Retroviral insertion into a host genome is a powerful tool not only for the discovery of cancer genes, but also for the discovery of potential oncogenic noncoding RNAs. In a large-scale mouse T lymphocyte tumo...
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Article
Open AccessSlow, stochastic transgene repression with properties of a timer
When gene expression varies unpredictably between genetically identical organisms, this is sometimes ascribed as stochastic. With the prevalence of retroviral vectors, stochastic repression is often observed a...
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Article
Mutational activity in cell line WEHI-231
The cell line WEHI-231 expresses activation-induced cytidine deaminase (AID), the enzyme that mediates hypermutation and immunoglobulin class switch recombination in activated B cells. Although both the cDNA s...
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Article
Autoreactivity and allelic inclusion in a B cell nuclear transfer mouse
Lymphocytes typically express only one functional antigen receptor, a restriction contributed to by allelic exclusion. Here we have analyzed B lymphocyte development in offspring of a mouse generated by nuclea...
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Article
Open AccessA murine leukemia virus with Cre-LoxP excisible coding sequences allowing superinfection, transgene delivery, and generation of host genomic deletions
To generate a replication-competent retrovirus that could be conditionally inactivated, we flanked the viral genes of the Akv murine leukemia virus with LoxP sites. This provirus can delete its envelope gene b...
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Article
Physical map of the mouse λ light chain and related loci
The λ light chain genes and the related loci λ5, V preB 1 and V preB 2 are selectively expressed in the development of B ly...
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Chapter
Biochemical Studies of Class Switch Recombination
Higher eukaryotes produce immunoglobulins (lg) of different classes, which are defined by the constant region (C) of the heavy chain. In the mouse the heavy chains are designated μ, δ, γ3, γ1, γ2b, γ2a, ε and ...
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Chapter
Somatic Hypermutability
In his book “What is life?” which was so influential at the tinne, Erwin Schrödinger (1944) mused, “In orderte be suitable material for the work of natural selection, mutations must be rare events.” That is, stab...
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Article
Hypermutation in T cells questioned
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Article
Immunoglobulin variable gene segment V H 81X of the mouse is embedded in L1 transposon sequences
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Article
Allelic exclusion model questioned
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Article
Circular DNA is a product of the immunoglobulin class switch rearrangement
THE class of immunoglobulin is defined by the constant region of its heavy chain. When a B lymphocyte switches the class of heavy chain it produces, the constant region of µ-type heavy chain is replaced (reviewed...
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Chapter
Die genetische Grundlage der Antikörpervielfalt
Thukydides beschreibt eine nicht identifizierte Seuche, die Athen im Peloponesischen Krieg (430 v. Chr.) heimsuchte. Ein Viertel der Athener Landstreitkräfte starben. „Diejenigen, die sich von der Kr...
