33 Result(s)
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Shyamala Maheswaran
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Article
Open AccessModeling the novel SERD elacestrant in cultured fulvestrant-refractory HR-positive breast circulating tumor cells
Metastatic hormone receptor-positive (HR+) breast cancer initially responds to serial courses of endocrine therapy, but ultimately becomes refractory. Elacestrant, a new generation FDA-approved oral selective ...
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Article
Open AccessDownregulation of KEAP1 in melanoma promotes resistance to immune checkpoint blockade
Immune checkpoint blockade (ICB) has demonstrated efficacy in patients with melanoma, but many exhibit poor responses. Using single cell RNA sequencing of melanoma patient-derived circulating tumor cells (CTCs...
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Protocol
Negative-Selection Enrichment of Circulating Tumor Cells from Peripheral Blood Using the Microfluidic CTC-iChip
The ability to isolate and analyze rare circulating tumor cells (CTCs) holds the potential to increase our understanding of cancer evolution and allows monitoring of disease and therapeutic responses through a...
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Article
Evaluation of endocrine resistance using ESR1 genoty** of circulating tumor cells and plasma DNA
Therapeutic efficacy of hormonal therapies to target estrogen receptor (ER)-positive breast cancer is limited by the acquisition of ligand-independent ESR1 mutations, which confer treatment resistance to aromatas...
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Article
Open AccessHIF1A signaling selectively supports proliferation of breast cancer in the brain
Blood-borne metastasis to the brain is a major complication of breast cancer, but cellular pathways that enable cancer cells to selectively grow in the brain microenvironment are poorly understood. We find tha...
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Article
Open AccessPancreatic circulating tumor cell profiling identifies LIN28B as a metastasis driver and drug target
Pancreatic ductal adenocarcinoma (PDAC) lethality is due to metastatic dissemination. Characterization of rare, heterogeneous circulating tumor cells (CTCs) can provide insight into metastasis and guide develo...
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Article
Open AccessA post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells
Quiescence (G0) is a transient, cell cycle-arrested state. By entering G0, cancer cells survive unfavorable conditions such as chemotherapy and cause relapse. While G0 cells have been studied at the transcript...
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Chapter
Quantitative Analysis of Circulating Tumor Cells Using RNA-Based Digital Scoring
Circulating tumor cells (CTCs) provide valuable information about the molecular evolution of cancers, as they may initially respond and ultimately progress on therapy. As intact tumor cells isolated from the b...
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Chapter
Epithelial-Mesenchymal Plasticity in Circulating Tumor Cells, the Precursors of Metastasis
Circulating tumor cells offer an unprecedented window into the metastatic cascade, and to some extent can be considered as intermediates in the process of metastasis. They exhibit dynamic oscillations in epith...
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Article
Open AccessBlood-based monitoring identifies acquired and targetable driver HER2 mutations in endocrine-resistant metastatic breast cancer
Plasma genoty** identifies potentially actionable mutations at variable mutant allele frequencies, often admixed with multiple subclonal variants, highlighting the need for their clinical and functional vali...
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Article
Open AccessSETD1A protects from senescence through regulation of the mitotic gene expression program
SETD1A, a Set1/COMPASS family member maintaining histone-H3-lysine-4 (H3K4) methylation on transcriptionally active promoters, is overexpressed in breast cancer. Here, we show that SETD1A supports mitotic process...
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Article
Open AccessRelationship between hepatocellular carcinoma circulating tumor cells and tumor volume
Microfluidic platforms have demonstrated the ability to isolate rare circulating tumor cells from a wide variety of cancers. Our group has recently shown the ability to isolate circulating tumor cells (CTCs) f...
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Article
Open AccessWhole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
Precise rare-cell technologies require the blood to be processed immediately or be stabilized with fixatives. Such restrictions limit the translation of circulating tumor cell (CTC)-based liquid biopsy assays ...
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Article
Open AccessMonolithic Chip for High-throughput Blood Cell Depletion to Sort Rare Circulating Tumor Cells
Circulating tumor cells (CTCs) are a treasure trove of information regarding the location, type and stage of cancer and are being pursued as both a diagnostic target and a means of guiding personalized treatme...
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Article
Open AccessPreservative solution that stabilizes erythrocyte morphology and leukocyte viability under ambient conditions
The deterioration of whole blood ex vivo represents a logistical hurdle in clinical and research settings. Here, a cocktail preservative is described that stabilizes leukocyte viability and erythrocyte morphology...
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Article
Open AccessMicrofluidic Isolation of Circulating Tumor Cell Clusters by Size and Asymmetry
Circulating tumor cell clusters (CTC clusters) are potent initiators of metastasis and potentially useful clinical markers for patients with cancer. Although there are numerous devices developed to isolate ind...
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Article
Open AccessExpression of β-globin by cancer cells promotes cell survival during blood-borne dissemination
Metastasis-competent circulating tumour cells (CTCs) experience oxidative stress in the bloodstream, but their survival mechanisms are not well defined. Here, comparing single-cell RNA-Seq profiles of CTCs fro...
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Article
HER2 expression identifies dynamic functional states within circulating breast cancer cells
Patient-derived circulating tumour cells are used to characterize the dynamics and underlying plasticity of HER2 expression in non-HER2-amplified breast tumours.
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Article
Open AccessThe Role of Physical Stabilization in Whole Blood Preservation
The rapid degradation of blood ex vivo imposes logistical limitations on the utilization of blood-borne cells in medical diagnostics and scientific investigations. A fundamental but overlooked aspect in the stora...
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Article
Open AccessDeformability of Tumor Cells versus Blood Cells
The potential for circulating tumor cells (CTCs) to elucidate the process of cancer metastasis and inform clinical decision-making has made their isolation of great importance. However, CTCs are rare in the bl...
