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Article
Open AccessReal-time monitoring of mitochondrial oxygenation during machine perfusion using resonance Raman spectroscopy predicts organ function
Organ transplantation is a life-saving procedure affecting over 100,000 people on the transplant waitlist. Ischemia reperfusion injury (IRI) is a major challenge in the field as it can cause post-transplantati...
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Article
Open AccessNovel Imaging Technologies for Accurate Assessment of Cardiac Allograft Performance
The current lack of objective and quantitative assessment techniques to determine cardiac graft relative viability results in risk-averse decision-making, which negatively impact the utilization of cardiac gra...
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Article
Open AccessPartial freezing of rat livers extends preservation time by 5-fold
The limited preservation duration of organs has contributed to the shortage of organs for transplantation. Recently, a tripling of the storage duration was achieved with supercooling, which relies on temperatu...
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Article
Open AccessThe effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion
In transplantation, livers are transported to recipients using static cold storage (SCS), whereby livers are exposed to cold ischemic injury that contribute to post-transplant risk factors. We hypothesized tha...
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Article
Epigenetic regulation by DNA methyltransferases during torpor in the thirteen-lined ground squirrel Ictidomys tridecemlineatus
The thirteen-lined ground squirrel, Ictidomys tridecemlineatus, is a mammal capable of lowering its Tb to almost 0 °C while undergoing deep torpor bouts over the winter. To decrease its metabolic rate to such a d...
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Article
Subzero non-frozen preservation of human livers in the supercooled state
Preservation of human organs at subzero temperatures has been an elusive goal for decades. The major complication hindering successful subzero preservation is the formation of ice at temperatures below freezin...
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Article
Open AccessMegakaryocytes contain extranuclear histones and may be a source of platelet-associated histones during sepsis
Histones are typically located within the intracellular compartment, and more specifically, within the nucleus. When histones are located within the extracellular compartment, they change roles and become dama...
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Article
Open AccessCell release during perfusion reflects cold ischemic injury in rat livers
The global shortage of donor organs has made it crucial to deeply understand and better predict donor liver viability. However, biomarkers that effectively assess viability of marginal grafts for organ transpl...
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Article
Supercooling extends preservation time of human livers
The inability to preserve vascular organs beyond several hours contributes to the scarcity of organs for transplantation1,2. Standard hypothermic preservation at +4 °C (refs. 1,3) limits liver preservation to les...
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Article
Stress-induced antioxidant defense and protein chaperone response in the freeze-tolerant wood frog Rana sylvatica
Freeze tolerance is an adaptive response utilized by the wood frog Rana sylvatica to endure the sub-zero temperatures of winter. Survival of whole body freezing requires wood frogs to trigger cryoprotective mecha...
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Article
Open AccessWhole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
Precise rare-cell technologies require the blood to be processed immediately or be stabilized with fixatives. Such restrictions limit the translation of circulating tumor cell (CTC)-based liquid biopsy assays ...
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Article
Response of the JAK-STAT pathway to mammalian hibernation in 13-lined ground squirrel striated muscle
Over the course of the torpor-arousal cycle, hibernators must make behavioral, physiological, and molecular rearrangements in order to keep a very low metabolic rate and retain organ viability. 13-lined ground...
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Article
The involvement of mRNA processing factors TIA-1, TIAR, and PABP-1 during mammalian hibernation
Mammalian hibernators survive low body temperatures, ischemia–reperfusion, and restricted nutritional resources via global reductions in energy-expensive cellular processes and selective increases in stress pa...
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Article
To be or not to be: the regulation of mRNA fate as a survival strategy during mammalian hibernation
Mammalian hibernators undergo profound behavioral, physiological, and biochemical changes in order to cope with hypothermia, ischemia-reperfusion, and finite fuel reserves over days or weeks of continuous torp...
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Article
Characterization of adipocyte stress response pathways during hibernation in thirteen-lined ground squirrels
To avoid the harsh conditions of winter climates, hibernating mammals undergo a systematic depression of physiological function by reducing their metabolic rate. During this process, hibernators are exposed to...
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Article
Structural and Functional Properties of Glycerol-3-Phosphate Dehydrogenase from a Mammalian Hibernator
Glycerol-3-phosphate dehydrogenase (G3PDH; E.C.1.1.1.8) was purified from liver and skeletal muscle of black-tailed prairie dogs (Cynomys ludivicianus), a hibernating species. Native and subunit molecular masses ...
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Article
Expression of myocyte enhancer factor-2 and downstream genes in ground squirrel skeletal muscle during hibernation
Myocyte enhancer factor-2 (MEF2) transcription factors regulate the expression of a variety of genes encoding contractile proteins and other proteins associated with muscle performance. We proposed that change...
