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Reference Work Entry In depth
Organic Matter BOD Biosensor Monitoring
From a proposal of a microbial Biochemical Oxygen Demand (BOD) biosensor by Karube et al. in 1977, components (recognition elements, transducer, etc.) have so far been developed from various perspectives such ...
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Living Reference Work Entry In depth
Organic Matter BOD Biosensor Monitoring
From a proposal of a microbial Biochemical Oxygen Demand (BOD) biosensor by Karube et al. in 1977, components (recognition elements, transducer, etc.) have so far been developed from various perspectives such ...
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Article
Open AccessIdentification of G-quadruplex clusters by high-throughput sequencing of whole-genome amplified products with a G-quadruplex ligand
G-quadruplex (G4) is a DNA secondary structure that has been found to play regulatory roles in the genome. The identification of G4-forming sequences is important to study the specific structure-function relat...
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Article
Open AccessIdentification of G-quadruplex structures that possess transcriptional regulating functions in the Dele and Cdc6 CpG islands
G-quadruplex is a DNA secondary structure that has been shown to play an important role in biological systems. In a previous study, we identified 1998 G-quadruplex-forming sequences using a mouse CpG islands D...
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Chapter
Yeast Based Sensors
Since the first microbial cell sensor was studied by Karube et al. in 1977, many types of yeast based sensors have been developed as analytical tools. Yeasts are known as facultative anaerobes. Facultative ana...
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Chapter
Applications of Microbial Cell Sensors
Since the first microbial cell sensor was studied by Karube et al. in 1977, many types of microbial cell sensors have been developed as analytical tools. The microbial cell sensor utilizes microbes as a sensin...
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Chapter
Development of Microbial Sensors and Their Application
Many types of microbial sensors have been developed as analytical tools since the first microbial sensor was studied by Karube et al. in 1977. The microbial sensor consists of a transducer and microbe ...
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Article
PCR-Based Ribosomal DNA Detection Technique for Microalga (Heterosigma carterae) Causing Red Tide and Its Application to a Biosensor Using Labeled Probe
A technique for detecting Raphidophycean, a bloom-forming genus of algae, was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the small subunit (SSU) ribo...
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Article
Current research activity in biosensors
Biosensors consist of a molecular recognition element and a transducer. Since the first biosensor was developed by Updike and Hicks [1] many biosensors and their associated techniques have been studied and dev...
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Article
Fructose-2,6-bisphosphate contents were increased in response to salt, water and osmotic stress in leaves of Bruguiera gymnorrhiza by differential changes in the activity of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphate 2-phosphatase
The steady-state level of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphate 2-phosphatase (F6P2K/F26BPase) transcript has been found to be raised in the mangrove Bruguiera gymnorrhiza treated with 500 mM NaCl fo...
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Article
Detection technique of asymmetric RT-PCR-based amplified single-stranded DNA and its application to biosensor for detection of mRNA for cyanobacteria, Anabaena variabilis
A real-time detection technique for mRNA was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the expressed mRNA of Anabaena variabilis PCC 7120 by using t...
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Article
Molecular cloning and characterization of genes encoding BURP domain-containing protein in the mangrove, Bruguiera gymnorrhiza
We cloned and characterized four independent genes encoding BURP domain-containing proteins from mangrove, Bruguiera gymnorrhiza, two as cDNA (BgBDC2 and BgBDC3) and the other two as genomic DNA (S2 and L3). The...
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Article
Molecular characterization and response to salt stress of mRNAs encoding cytosolic Cu/Zn superoxide dismutase and catalase from Bruguiera gymnorrhiza
To analyze the potential of the active oxygen-scavenging system of the cytosol in leaves of salt-stressed Bruguiera gymnorrhiza, we isolated a full-length cDNA encoding a 153-amino-acid sequence of cytosolic Cu/...
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Article
Observation and Application of a Pattern in a DNA-Histone Mixture
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Article
Isolation of a bacterium from mangrove soil for degradation of sea sludge
Sea sludge, which is sediment of fish excrement and sewage on the sea bottom, continues to be a serious environmental problem. It has the potential to cause eutrophication and red tide, resulting in the death ...
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Article
Sensor Chip Using a Plasma-polymerized Film for Surface Plasmon Resonance Biosensors: Reliable Analysis of Binding Kinetics
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Article
Multifunctional Biocompatible Membrane and Its Application to Fabricate A Miniaturized Glucose Sensor with Potential for Use In Vivo
A multifunctional membrane with biocompatibility, diffusion-limiting effect, and the ability to curtail the responses of an H2O2 electrode to ascorbate and urate was prepared. It was composed of MB, AB, and CTA, ...
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Article
Eicosapentaenoic acid release from the red alga Pachymeniopsis lanceolata by enzymatic degradation
Forty-eight species of seaweeds from Japanese waters were screened for the valuable polyunsaturated fatty acids eicosapentaenoic acid (EPA). The eight species that contained the highest levels of these compoun...
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Article
Purification of single stranded DNA from asymmetric PCR product using the SMART system
A method has been developed using the SMART system for the purification of single stranded DNA from a mixture containing single- and double-stranded DNA amplified using asymmetric PCR. The asymmetric PCR produ...
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Article
Enhanced eicosapentaenoic acid production by Navicula saprophila
Changes in the eicosapentaenoic acid (EPA) content of Navicula saprophila grown photoautotrophically and mixotrophically with the addition of acetate were examined in terms of their growth progress. Among four...
