Abstract
Forty-eight species of seaweeds from Japanese waters were screened for the valuable polyunsaturated fatty acids eicosapentaenoic acid (EPA). The eight species that contained the highest levels of these compounds were analyzed in detail. Of all species tested the red alga Pachymeniopsis lanceolata contained the highest EPA concentration, and it was present as both the free and bound forms. EPA constituted 38.7% of total fatty acids, and polar lipids were the main constituent of the total lipids in P. lanceolata. EPA was obtained from the marine algae P. lanceolata by enzymatic hydrolysis of the total lipids extract using phospholipase A2(PLA2). The release of EPA reached a plateau after 10 min of enzymatic treatment. These results suggest that P. lanceolata is a useful natural source of EPA and that PLA2 treatment is a convenient method for obtaining EPA from the red alga.
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Thorngren, M. and Gustafson, A. (1981), Lancet 2, 1190–1193.
Croset, M., Vericel, E., Rigaud, M., Hanss, M., Courpron, P., Dechavanne, M., and Lagarde, M. (1990), Thromb. Res. 57, 1–12.
Clubb, F. J., Jr., Schmitz, J. M., Butler, M. M., Buja, L. M., Willerson, J. T., and Campbell, W. B. (1989), Arteriosclerosis 9, 529–537.
Schmidt, E. B., Varming, K., Svaneborg, N., and Dyerberg, J. (1992), Ann. Nutr. Metab. 36, 283–287.
Urakaze, M., Hamazaki, T., Soda, Y., Miyamoto, M., Ibuki, F., Yano, S., and Kumagai, A. (1986), Thromb. Res. 44, 673–682.
Wojenski, C. M., Silver, M. J., and Walker, J. (1991), Biochim. Biophys. Acta 1081, 33–38.
Tamura, Y., Hirai, A., Terano, T., Yoshida, S. Takenaga, M., and Kitagawa, H. (1987), Jpn. Circ. J. 51, 471–477.
Tsuruta, K., Ogawa, H., Yasue, H., Sakamoto, T., Miyao, Y., Tanae, H., and Kaiga, K. (1996), Coron. Artery Dis. 7, 837–842.
Shinozaki, K., Kambayashi, J., Kawasaki, T., Uemura, Y., Sakon, M., Shiba, E., Shibuya, T., Nakamura, T., and Mori, T. (1996), J. Atheroscler. Thromb. 2, 107–109.
Lopez, A. D., Molina, G. E., Sanchez, P. J. A., Garcia, S. J. L., and Garcia, C. F. (1992), Aquaculture 102, 363–371.
Yongmanitchai, W. and Ward, O. P. (1991), Phytochemistry 30, 2963–2968.
Cohen, Z. and Cohen, S. (1991), J. Am. Oil Chem. Soc. 68, 16–19.
Sanchez, P. J. A. (1994), J. Appl. Phycol. 6, 475–478.
Yongmanitchai, W. and Ward, O. P. (1991), Appl. Environ. Microbiol. 57, 419–425.
Ohta, S., Chang, T., Aozasa, O., Kondo, M., and Miyata, H. (1992), J. Ferm. Bioeng. 74, 398–402.
Dembitsky, V. M., Pechenkina, S. E. E., and Rozentsvet, O. A. (1991), Phytochemistry 30, 2279–2284.
Fleurence, J., Gutbier, G., Mabeau, S., and Leray, C. (1994), J. Appl. Phycol. 6, 527–532.
Hatakeda, K., Ito, S., Sato, O., Saito, N., Ikushima, Y., and Goto, T. (1993), The Chem. Soc. Jpn. 4, 408–409.
Folch, J., Lees, M., and Sloan-Stanley, G. H. (1957), J. Biol. Chem. 226, 497–509.
Shuster, C. Y., Froines, J. R., and Olcott, H. S. (1964), J. Am. Oil Chem. Soc. 41, 36–41.
Ryu, E. K. and MacCoss, M. (1979), J. Lipid Res. 20, 561–563.
Norman, H. A. and Thompson, G. A. Jr. (1985), Arch. Biochem. Biophys. 242, 168–175.
Springer, M., Franke, H., and Pulz, O. (1994), J. Plant Physiol. 143, 534–537.
Cossins, A. R. (1994), Temperature Adaptation of Biological Membranes, Portland Press, London.
Los, D. A., Ray, M. K., and Murata, N. (1997), Mol. Microbiol. 25, 1167–1175.
Sakamoto, T., Shen, G., Higashi, S., Murata, N., and Bryant, D. A. (1998), Arch. Microbiol. 169, 20–28.
Niima, Y. and Taguchi, S. (1966), Nippon Suisan Gakkaisi 32, 1037–1042.
Ando, H. and Kaneda, N. (1968), Eiyou to Shokuryou 21, 245–249.
Molina, G. E., Sanchez, P. J. A., Garcia, C. F., Garcia, S. J. L., and Fernandez, S. J. M. (1995), J. Appl. Phycol. 7, 129–134.
Reis, A., Gouveia, L., Veloso, V., Fernandes, H. L., Empis, J. A., and Novais, J. M. (1996), Biores. Technol. 55, 83–88.
Otero, A., Garcia, D., Morales, E. D., Aran, J., and Fabregas, J. (1997), Biotechnol. Appl. Biochem. 26, 171–177.
Kitano, M., Matsukawa, R., and Karube, I. (1998), J. Appl. Phycol. 10, 101–105.
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Matsukawa, R., Hatakeda, K., Ito, S. et al. Eicosapentaenoic acid release from the red alga Pachymeniopsis lanceolata by enzymatic degradation. Appl Biochem Biotechnol 80, 141–150 (1999). https://doi.org/10.1385/ABAB:80:2:141
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DOI: https://doi.org/10.1385/ABAB:80:2:141