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Purification of single stranded DNA from asymmetric PCR product using the SMART system

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Biotechnology Techniques

Abstract

A method has been developed using the SMART system for the purification of single stranded DNA from a mixture containing single- and double-stranded DNA amplified using asymmetric PCR. The asymmetric PCR product was separated into single- and double-stranded DNA using an anion exchange column which took 15 min. Compared to another method in which biotinylated symmetric PCR products were applied to an immobilized streptavidin column, this method was simple and could purify single- and double-stranded DNA. © Rapid Science Ltd. 1998

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Authors and Affiliations

  1. Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku Tokyo, Japan

    Eriko Kai, Koichi Sumikura, Kazunori Ikebukuro & Isao Karube

Authors
  1. Eriko Kai
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  2. Koichi Sumikura
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  3. Kazunori Ikebukuro
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  4. Isao Karube
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Kai, E., Sumikura, K., Ikebukuro, K. et al. Purification of single stranded DNA from asymmetric PCR product using the SMART system. Biotechnology Techniques 12, 935–939 (1998). https://doi.org/10.1023/A:1008829931836

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  • DOI: https://doi.org/10.1023/A:1008829931836

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