-
Article
Open AccessCell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells
Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or...
-
Article
Open AccessA method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall
Genome engineering in plants is highly dependent on the availability of effective molecular techniques. Despite vast quantities of research, genome engineering in plants is still limited in terms of gene deliv...
-
Protocol
Selection and Characterization of DNA Aptamers Against FokI Nuclease Domain
Genome editing with site-specific nucleases (SSNs) may be effective for gene therapy, as SSNs can modify target genes. However, the main limitation of genome editing for clinical use is off-target effects by e...
-
Article
Open AccessOscillating high-aspect-ratio monolithic silicon nanoneedle array enables efficient delivery of functional bio-macromolecules into living cells
Delivery of biomolecules with use of nanostructures has been previously reported. However, both efficient and high-throughput intracellular delivery has proved difficult to achieve. Here, we report a novel mat...
-
Chapter
Specialized Nanoneedles for Intracellular Analysis
Here, we introduce a novel approach to the detection of intracellular molecules by measuring direct interactions with an ultrathin probe, i.e. nanoneedle, which is mounted on an atomic force microscope (AFM). ...
-
Chapter
Combinatorially Developed Peptide Receptors for Biosensors
Various combinatorial libraries were screened for short peptides of 4–10 mer, which were used as sensor molecules for capturing target chemicals or biomolecules. Immuno-antibodies can be synthesized in the liv...
-
Article
Nanostructure Fabrication Based on Engineered α-Synuclein
The utilization of biomaterials such as proteins or peptides has recently been focused on as an attractive way to construct nanomaterials by “bottom-up” strategy. We focus on α-synuclein as a novel scaffold ma...
-
Article
Nanoneedle Surface Modification with 2-Methacryloyloxyethyl Phosphorylcholine Polymer to Reduce Nonspecific Protein Adsorption in a Living Cell
Previously, we developed a new molecular delivery system to target single living cells by using atomic force microscope and ultrathin needle referred to as nanoneedle. This system delivers molecules into the c...
-
Article
Electrochemical properties of hydrogenase on glass carbon electrodes modified with carbon nanotubes
Hydrogenase (H2 ase) purified from phototropic bacteriumThiocapsa roseopersicina was coassembled with carbon nanotubes (CNTs) on glass carbon electrodes. Both oxidized CNTs and Nafion-CNT composites were used to ...
-
Article
Analysis of DNA and Zinc finger interactions using mechanical force spectroscopy
The unbinding force of Zif268-DNA complex has been studied by atomic force microscopy (AFM). DNA and Zif268 were covalently immobilized on the surfaces of an AFM tip and glass substrate, respectively. Confocal...
-
Article
Direct insertion of proteins into a living cell using an atomic force microscope with a nanoneedle
We have developed a tool for directly inserting proteins into living cells by using atomic force microscopy (AFM) and an ultrathin needle, termed a nanoneedle. The surface of the nanoneedle was modified with H...
-
Article
PCR-Based Ribosomal DNA Detection Technique for Microalga (Heterosigma carterae) Causing Red Tide and Its Application to a Biosensor Using Labeled Probe
A technique for detecting Raphidophycean, a bloom-forming genus of algae, was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the small subunit (SSU) ribo...
-
Article
Detection technique of asymmetric RT-PCR-based amplified single-stranded DNA and its application to biosensor for detection of mRNA for cyanobacteria, Anabaena variabilis
A real-time detection technique for mRNA was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the expressed mRNA of Anabaena variabilis PCC 7120 by using t...
-
Article
Direct triazine herbicide detection using a self-assembled photosynthetic reaction center from purple bacterium
In this study, a direct detection system for triazine derivative herbicides was developed using the photosynthetic reaction center (RC) from the purple bacterium,Rhodobacter sphaeroides, and surface plasmon reson...
-
Article
Fabrication of an electrode-viologen-hydrogenase heterogeneous system and the electrochemical hydrogen evolution
An indium tinoxide (ITO) electrode was chemically modified by one layer of viologen (VIO) deri vative, which possessed a persistent and reproducible electrochemical response. A monolayer of a thermal stable hy...
-
Article
Self-assembling photosynthetic reaction centers on electrodes for current generation
Photosynthetic reaction centers (RCs) made from photosynthetic organisms can be used in solar batteries because their molecules cause light-induced charge separation. We present a simple immobilization system ...
-
Chapter
Self-Assembling Photosynthetic Reaction Centers on Electrodes for Current Generation
Photosynthetic reaction centers (RCs) made from photosynthetic organisms can be used in solar batteries because their molecules cause light-induced charge separation. We present a simple immobilization system ...
-
Chapter
Fabrication of an Electrode-Viologen-Hydrogenase Heterogeneous System and the Electrochemical Hydrogen Evolution
An indium tin oxide (ITO) electrode was chemically modified by one layer of viologen (VIO) derivative, which possessed a persistent and reproducible electrochemical response. A monolayer of a thermal stable hy...
-
Article
Expression of luciferase gene under control of the puf promoter from Rhodobacter sphaeroides
An expression vector for purple bacteria based on the puf promoter from Rhodobacter sphaeroides was constructed. The 1.2 kb promoter fragment included the pufQ gene, ORFK, as wellas the SD sequence and thestartco...
-
Article
Isolation and analysis of tetraheme-bound-cytochrome from photosynthetic reaction centers of Rhodopseudomonas viridis
A tetraheme cytochrome (BCytc) was isolated from the photosynthetic reaction centers (RC) of Rhodopseudomonas viridis while maintaining the redox activity. BCytc was removed from the H-subunit-detached RC by poly...
