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Protocol
Microbial biosensors based on respiratory inhibition
Biosensors generally provide a rapid and convenient alternative to conventional methods for monitoring chemical substances in fields as diverse as medicine, environmental monitoring, and food processing. A bio...
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Article
Changes in eicosapentaenoic acid content of Navicula saprophila, Rhodomonas salina and Nitzschia sp. under mixotrophic conditions
Three species of microalgae able to produce eicosapentaenoic acid(EPA) were collected from brackish and sea water around Japan. The species were identified as Navicula saprophila, Rhodomonassalina and Nitzschi...
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Article
Enzymatic screening of microalgae as a potential source of natural antioxidants
Extracts of over 100 species of microalgae both from nature and from laboratory cultures were screened for antioxidant activity. As an assay for antioxidant function, we examined the inhibition of the activity...
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Article
Photo-induced activation of cytochrome P450/reductase fusion enzyme coupled with spinach chloroplasts
Photoactivation of cytochrome P450 monooxygenase was studied using a combination of spinach chloroplasts and yeast microsomes containing rat P4501A1/yeast reductase fusion enzyme. Under illumination, in the re...
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Article
Determination of cyanide using a microbial sensor
A microbial cyanide sensor was prepared, consisting of immobilizedSaccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of ...
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Article
A novel synthesis method for cyclodextrins from maltose in water-organic solvent systems
A novel enzymatic synthesis method of cyclodextrin (CD) from low-mol-wt maltose using cyclomaltodextrin glucanotransferase (CGTase) fromBacillus macerans has been developed in various water-organic solvent system...
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Article
Enzymatic hydrolysis of starch in water-immiscible organic solvent, two-phase systems
Enzyme-catalyzed hydrolyzations of starch by α-amylase have been studied in various two-phase systems, consisting of water and a water-immiscible organic solvent. The hydrolytic conversion of soluble starch to...
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Article
Continuous two step bioconversion of 4-oxoiso-phorone (OIP) to 4-hydroxy-2,2,6-trimethylcyclo-hexanone (4-HTMCH) by thermophilic bacteria
The continuous two step conversion of 4-oxoisophorone(OIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone(4-HTMCH) via dihydrooxoisophorone(DOIP) was carried out using two types of thermophilic growing cells, Thermomo...
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Article
In vivo fluorometric method for early detection of cyanobacterial waterblooms
A specific method was developed for monitoring the concentration of cyanobacteria (blue-green algae) before waterblooms, based on their characteristics ofin vivo fluorescence. The excitation and emission spectra ...
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Article
Influence of cationic antibiotics on phase behavior of rough-form lipopolysaccharide
The rough-form lipopolysaccharide (LPS) interacted with cationic antibiotic polymyxin B and gramicidin S in solution, and showed altered thermotropic phase behavior and viscoelasticity. The phase behavior was ...
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Chapter and Conference Paper
Chemical Vapor Sensor Using a Lipid-Coated SAW Resonator Oscillator
The sensitivity of piezoelectric Detectors and surface acoustic wave (SAW) devices is directly proportional to the square of the resonant frequency, and is inversely proportional to the surface area. Therefore...
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Article
Research and development of biosensors in Japan
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Chapter and Conference Paper
Development of Micro—Biosensors for Brain Research
Neurotransmitters play important roles in the brain since they are the key link in communication between neurons. Acetylcholine is the transmitter of motor neurons in the spinal cord as well as in all the nerv...
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Chapter
Microbial Sensors and Microbiosensors
A novel BOD sensor with a new principle employing a luminous bacterium, Photobacterium phosphoreum, was developed. The increased intensity of luminescence resulting from the cellular assimilation of organic compo...
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Article
A biocompatible needle-type glucose sensor based on platinum-electroplated carbon electrode
A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a d...
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Article
Structural characterization of lipid A component of Erwinia carotovora lipopolysaccharide
The chemical structure of the lipid A component of lipopolysaccharide excreted into the liquid medium by the plant pathogenic enterobacterium Erwinia carotovora FERM P-7576 was characterized. It consists of a β-1...
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Article
Development of New Microbiosensors
The miniaturization of biosensors is very promising in various field of applications. The authors have been develo** many kinds of micro biosensors based on micro devices fabricated by semiconductor fabricat...
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Article
Amplification immunoassay for the determination of Hepatitis B surface antigen
A sensitive sandwich immunoassay for the determination of Hepatitis B surface antigen (HBs) was developed, using a cascade system of Limulus amebocyte lysate as a signal amplification system. Lipo-polysacchari...
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Article
Facile isolation ofEndo-pectate lyase fromErwinia carotovora based on electrostatic interaction
Endo-pectate lyase (PATE) fromErwinia carotovora was selectively cosedimented with extracellularly produced lipopolysaccharide-lipid complex (LPSLC) through dialysis of the cell free culture broth. The selective ...
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Article
Sequential two-step conversion of 4-oxoisophorone to 4-hydroxy-2,2,6-trimethylcyclohexanone by thermophilic bacteria
The sequential two-step conversion of 4-oxoisophorone (OIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone (4-HTMCH) via diyhdrooxoisophorone (DOIP) was achieved using two kinds of thermophiles, Thermomonospora curvat...
