69 Result(s)
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Protocol
Host Factors Modulating RSV Infection: Use of Small Interfering RNAs to Probe Functional Importance
Although respiratory syncytial virus (RSV) is the leading cause of bronchiolitis and pneumonia in infants and the elderly worldwide [1], the protein–protein interactions between the host cell and virus remain ...
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Protocol
Automated Cell-by-Cell Tissue Imaging and Single-Cell Analysis for Targeted Morphologies by Laser Ablation Electrospray Ionization Mass Spectrometry
Mass spectrometry imaging (MSI) is an emerging technology for the map** of molecular distributions in tissues. In most of the existing studies, imaging is performed by sampling on a predefined rectangular gr...
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Protocol
MALDI-MS-Assisted Molecular Imaging of Metabolites in Legume Plants
Mass spectrometric imaging (MSI) is a powerful analytical tool that provides spatial information of several compounds in a single experiment. This technique has been used extensively to study proteins, peptide...
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Protocol
Low Magnification Confocal Microscopy of Tumor Angiogenesis
Blood vessels are critical to normal mammalian development, tissue repair, and growth and treatment of cancer. Mouse research models enable mechanistic studies of blood vessels. We detail how to perfuse mice w...
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Protocol
siRNA Screens Using Drosophila Cells to Identify Host Factors Required for Infection
Drosophila melanogaster offers a powerful model system for interrogating interactions between host cells and human bacterial pathogens. Brucella, a gram-negative, facultative intracellular bacter...
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Protocol
Electron Spectroscopic Tomography of Specific Chromatin Domains
The eukaryotic genome is packaged within the nucleus as poly-nucleosome 10 nm chromatin fibres. The nucleosome core particle, the fundamental chromatin subunit, consists of a DNA molecule wrapped around a hist...
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Protocol
Atomic Force Microscopy of Plasmodium-Infected Red Blood Cells: Detecting and Localizing Single Molecular Recognition Events
Atomic Force Microscopy (AFM) is a powerful tool for exploring the interaction between ligands and receptors, as well as their exact locations on the red cell surface. Here we discuss current and future applic...
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Protocol for the Use of Light Upon Extension Real-Time PCR for the Determination of Viral Load in HBV Infection
Real-time PCR has engendered wide acceptance for quantitation of hepatitis B virus (HBV) DNA in the blood due to its improved rapidity, sensitivity, reproducibility, and reduced contamination. Here we describe...
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Protocol for the Use of a Bead Array for the Multiple Detection of Genotype of Chlamydia trachomatis
The identification of Chlamydia trachomatis genotypes is important for both molecular epidemiology and infection control such as contact tracing and identification of high-risk groups. Currently, at least 19 huma...
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Protocol
Systems Biology Approach to Imaging of Neural Stem Cells
Over the past decade, the advances in human brain magnetic resonance imaging (MRI) have significantly improved our ability to gain insightful information about the structure and function of the brain. One of t...
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Protocol
Atomic Force Microscopy as Nanorobot
Atomic force microscopy (AFM) is a powerful and widely used imaging technique that can visualize single molecules under physiological condition at the nanometer scale. In this chapter, an AFM-based nanorobot f...
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Protocol
Synthetic Fluorescent Probes for Imaging of Peroxynitrite and Hypochlorous Acid in Living Cells
Peroxynitrite (ONOO–) and hypochlorous acid (HOCl) are two highly reactive oxygen species generated in biological systems. The overproduction of peroxynitrite or hypochlorous acid is implicated in a broad array o...
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Protocol
s-RT-MELT: A Novel Technology for Mutation Screening
The fast growing understanding of genetic pathways that mediate cancer etiology, biology, and personalized medicine leads to an increasing need for extensive and reliable mutation screening on a population or ...
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Protocol
Photo-Activatable Probes for the Analysis of Receptor Function in Living Cells
Photo-activatable (caged) probes are powerful research tools for biological investigation. The superb maneuverability of a light beam allows researchers to activate caged probes with pinpoint accuracy. Recent ...
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Protocol
Digital Candidate Gene Approach (DigiCGA) for Identification of Cancer Genes
The candidate gene approach is one of the most commonly used methods for identifying genes underlying disease traits. Advances in genomics have greatly contributed to the development of this approach in the pa...
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Protocol
In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells
Transdifferentiation is defined as an irreversible switch in postnatal life of one differentiated cell to another. Transdifferentiation from different cellular origins into pancreatic-like β-cells is of clinic...
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Protocol
Identification of Human Pancreatic Cancer Stem Cells
Emerging evidence suggests that malignant tumors are composed of a small subset of distinct cancer cells, termed “cancer stem cells” (typically less than 5% of total cancer cells based on cell surface marker e...
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Protocol
Molecular Analysis of Genetic Instability Caused by Chronic Inflammation
Genetic instability is a hallmark of human cancers. It is the driving force for tumor development as it facilitates the accumulation of mutations in genes that regulate cell death and proliferation and therefo...
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Protocol
iTRAQ-Based Shotgun Neuroproteomics
Shotgun proteomics involves the analysis of peptides obtained by enzymatic digestions of proteins and subsequent identification via tandem mass spectrometry. This approach is an effective method for studying g...
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Protocol
Computing Genetic Imprinting Expressed by Haplotypes
Different expression of maternally and paternally inherited alleles at certain genes is called genetic imprinting. Despite its great importance in trait formation, development, and evolution, it remains unclea...
