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Article
Open AccessAuthor Correction: Protective antibody response following oral vaccination with microencapsulated Bacillus Anthracis Sterne strain 34F2 spores
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Article
Open AccessProtective antibody response following oral vaccination with microencapsulated Bacillus Anthracis Sterne strain 34F2 spores
An oral vaccine against anthrax (Bacillus anthracis) is urgently needed to prevent annual anthrax outbreaks that are causing catastrophic losses in free-ranging livestock and wildlife worldwide. The Sterne vaccin...
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Article
Human Brucellosis and Adverse Pregnancy Outcomes
Brucellosis is a neglected, zoonotic disease of nearly worldwide distribution. Despite brucellosis being recognized as a reproductive disease in animals, it has been historically known as a flu-like illness in...
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Protocol
siRNA Screens Using Drosophila Cells to Identify Host Factors Required for Infection
Drosophila melanogaster offers a powerful model system for interrogating interactions between host cells and human bacterial pathogens. Brucella, a gram-negative, facultative intracellular bacter...
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Article
Open AccessBrucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression
Quorum sensing is a communication system that regulates gene expression in response to population density and often regulates virulence determinants. Deletion of the luxR homologue vjbR highly attenuates intracel...
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Protocol
In Vitro Mutagenesis of Brucella Species
Three major techniques have been employed for broad-range in vitro mutagenesis of Brucella species. Shotgun approaches capable of generating large libraries of randomly inserted transposon mutants include Tn5, ma...
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Chapter
Brucella
Brucellosis affects millions of animals and humans world-wide; in humans, over 500,000 new cases are reported annually. Although some vaccines are available for its prevention in animals, none exist for humans...
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Article
Open AccessMariner mutagenesis of Brucella melitensis reveals genes with previously uncharacterized roles in virulence and survival
Random gene inactivation used to identify cellular functions associated with virulence and survival of Brucella spp has relied heavily upon the use of the transposon Tn5 that integrates at G/C base pairs. Transpo...
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Chapter
Of Mice, Calves, and Men
Numerous Salmonella typhimurium virulence factors have been identified and characterized using experimental infection of mice. While the murine typhoid model has been used successfully for Salmonella typhi vaccin...
