16 Result(s)

Chapter

Minor Allele Enrichment in Liquid Biopsies Using Nuclease-Assisted Elimination of Wild-Type DNA

Circulating DNA analysis presents opportunities in harnessing the potential of tumor biomarkers in a minimally invasive manner but also presents several technical challenges. For example, excess wild-type (WT)...

Ka Wai Leong, Fangyan Yu, G. Mike Makrigiorgos in Circulating Tumor Cells (2023)

Article

Massively parallel enrichment of low-frequency alleles enables duplex sequencing at low depth

Assaying for large numbers of low-frequency mutations requires sequencing at extremely high depth and accuracy. Increasing sequencing depth aids the detection of low-frequency mutations yet limits the number o...

Gregory Gydush, Erica Nguyen, ** H. Bae, Timothy Blewett… in Nature Biomedical Engineering (2022)

Protocol

Nuclease-Assisted, Multiplexed Minor-Allele Enrichment: Application in Liquid Biopsy of Cancer

The use of next-generation sequencing (NGS) to profile genomic variation of individual cancer species is revolutionizing the practice of clinical oncology. In liquid biopsy of cancer, sequencing of circulating...

Fangyan Yu, Ka Wai Leong, G. Mike Makrigiorgos in Biomedical Engineering Technologies (2022)

Chapter and Conference Paper

Nanoparticle-aided Radiotherapy for Retinoblastoma and Choroidal Melanoma

This work investigates the dosimetric feasibility of employing gold nanoparticles (AuNPs) or carboplatin nano- particles (CNPs) to enhance radiotherapy (RT) treatment efficacy for ocular cancers: retinoblastom...

Yucel Altundal, Erno Sajo… in World Congress on Medical Physics and Biom… (2015)

Article

Image-guided radiotherapy platform using single nodule conditional lung cancer mouse models

Close resemblance of murine and human trials is essential to achieve the best predictive value of animal-based translational cancer research. Kras-driven genetically engineered mouse models of non-small-cell lung...

Grit S. Herter-Sprie, Houari Korideck, Camilla L. Christensen… in Nature Communications (2014)

Protocol

COLD-PCR Enriches Low-Level Variant DNA Sequences and Increases the Sensitivity of Genetic Testing

Detection of low-level mutations is important for cancer biomarker and therapy targets discovery, but reliable detection remains a technical challenge. The newly developed method of CO-amplification at Lower D...

Elena Castellanos-Rizaldos, Coren A. Milbury… in Molecular Diagnostics for Melanoma (2014)

Article

NRAS mutations with low allele burden have independent prognostic significance for patients with lower risk myelodysplastic syndromes

D M Murphy, R Bejar, K Stevenson, D Neuberg, Y Shi, C Cubrich, K Richardson… in Leukemia (2013)

Article

Chitosan Film Containing Poly(D,L-Lactic-Co-Glycolic Acid) Nanoparticles: A Platform for Localized Dual-Drug Release

To characterize and evaluate chitosan film containing PLGA nanoparticles (NPs) as a platform for localized dual-drug release.

Dayane B. Tada, Surinder Singh, Dattatri Nagesha, Evan Jost… in Pharmaceutical Research (2010)

Protocol

s-RT-MELT: A Novel Technology for Mutation Screening

The fast growing understanding of genetic pathways that mediate cancer etiology, biology, and personalized medicine leads to an increasing need for extensive and reliable mutation screening on a population or ...

** Li, G. Mike Makrigiorgos in Cancer Susceptibility (2010)

Article

Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing

PCR is widely employed as the initial DNA amplification step for genetic testing. However, a key limitation of PCR-based methods is the inability to selectively amplify low levels of mutations in a wild-type b...

** Li, Lilin Wang, Harvey Mamon, Matthew H Kulke, Ross Berbeco… in Nature Medicine (2008)

Article

Anti-primer quenching-based real-time PCR for simplex or multiplex DNA quantification and single-nucleotide polymorphism genoty**

Nucleic acid amplification and detection plays an increasingly important role in genetic analysis of clinical samples, medical diagnostics and drug discovery. We present a new quantitative PCR method that allo...

** Li, G Mike Makrigiorgos in Nature Protocols (2007)

Article

A PCR-based amplification method retaining the quantitative difference between two complex genomes

With the increasing emergence of genome-wide analysis technologies (including comparative genomic hybridization (CGH)¹, expression profiling on microarrays², differential display (DD)³, subtractive hybridization,...

G. Mike Makrigiorgos, Subrata Chakrabarti, Yuzhi Zhang, Manjit Kaur… in Nature Biotechnology (2002)