Single Cell Metabolism
Methods and Protocols
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Article
Feature-based molecular networking in the GNPS analysis environment
Molecular networking has become a key method to visualize and annotate the chemical space in non-targeted mass spectrometry data. We present feature-based molecular networking (FBMN) as an analysis method in t...
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Article
Direct Drug Analysis in Polymeric Implants Using Desorption Electrospray Ionization – Mass Spectrometry Imaging (DESI-MSI)
Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) coupled with gas-phase ion mobility spectrometry was used to characterize the drug distribution in polymeric implants before and after ex...
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Book
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Protocol
Single-Cell Metabolomics by Mass Spectrometry
Single-cell level metabolomics gives a snapshot of small molecules, intermediates, and products of cellular metabolism within a biological system. These small molecules, typically less than 1 kDa in molecular ...
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Protocol
Spatial Map** of Cellular Metabolites Using DESI Ion Mobility Mass Spectrometry
Spatial map** of cellular metabolites, such as neurotransmitters and lipids, on the tissue, can increase our understanding of the biological functions of those molecules. Mass spectrometry imaging (MSI) tech...
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Protocol
Ten Major Future Challenges in Single-Cell Metabolomics
Information on cellular metabolism at the single-cell level can unravel countless biochemical process providing invaluable biomedical insight. Single-cell analysis field is at the very early stage at this mome...
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Protocol
Ion-Mobility Mass Spectrometry for Lipidomics Applications
Among lipidomics’ major challenges, there is the molecular complexity of the lipidome. State-of-the-art technology, such as ion mobility (IM) spectrometry, is a promising new tool for supporting lipidomics res...
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Article
Open AccessRapid Detection of Necrosis in Breast Cancer with Desorption Electrospray Ionization Mass Spectrometry
Identification of necrosis in tumors is of prognostic value in treatment planning, as necrosis is associated with aggressive forms of cancer and unfavourable outcomes. To facilitate rapid detection of necrosis...
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Chapter
Microprobe MS Imaging of Live Tissues, Cells, and Bacterial Colonies Using LAESI
Laser ablation electrospray ionization (LAESI) is an ambient ionization technique for mass spectrometry that is capable of performing direct spatial imaging on biological specimens with minimal-to-no sample pr...
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Protocol
Automated Cell-by-Cell Tissue Imaging and Single-Cell Analysis for Targeted Morphologies by Laser Ablation Electrospray Ionization Mass Spectrometry
Mass spectrometry imaging (MSI) is an emerging technology for the map** of molecular distributions in tissues. In most of the existing studies, imaging is performed by sampling on a predefined rectangular gr...
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Protocol
Relative Quantitation in Single-Cell Metabolomics by Laser Ablation Electrospray Mass Spectrometry
Single-cell analysis of metabolites by mass spectrometry (MS) is challenging due to the very limited volume and inherent molecular complexity of the sample. Quantitative metabolomic analysis of individual cell...
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Article
Open AccessDirect detection of diverse metabolic changes in virally transformed and Tax-expressing cells by mass spectrometry
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Article
Ablation and analysis of small cell populations and single cells by consecutive laser pulses
Laser ablation of single cells through a sharpened optical fiber is used for the detection of metabolites by laser ablation electrospray ionization (LAESI) mass spectrometry (MS). Ablation of the same Allium cepa
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Article
Molecular imaging by Mid-IR laser ablation mass spectrometry
Mid-IR laser ablation at atmospheric pressure (AP) produces a mixture of ions, neutrals, clusters, and particles with a size distribution extending into the nanoparticle range. Using external electric fields t...
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Article
Rapid analysis of pharmaceuticals and excreted xenobiotic and endogenous metabolites with atmospheric pressure infrared MALDI mass spectrometry
Atmospheric pressure (AP) infrared (IR) matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) was demonstrated for the rapid direct analysis of pharmaceuticals, and excreted human metaboli...
