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Antibacterial Activity of Postbiotics
Exploring natural antimicrobials in pharmaceutical and food industries has recently become popular, due to its increased demand, safety, and...
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Dispersive Liquid–Liquid Microextraction
Dispersive liquid–liquid microextraction (DLLME) was initially described as an effective sample preparation technology in 2006. However, researchers...
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Tyrosinase-mediated rapid and permanent chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogel
Chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogels were rapidly and stably prepared without any crosslinking materials by using an...
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Determination of Ochratoxin A in Plant Products: Immunoaffinity Column and Liquid Chromatography with Fluorescence Detector
Ochratoxins are mycotoxins produced by various fungi of the genus Aspergillus and Penicillium, e.g., A. ochraceus, A. carbonarius, and P. verrucosum....
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Gas Chromatography
Gas chromatography (GC) has many applications in the analysis of food products. A common GC application is fatty acid profile analysis, to meet the...
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Homogeneous Liquid–Liquid Microextraction
The development of liquid phase microextraction methods has led to significant progress in extraction processes by overcoming several challenges...
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Hydrogen generation derived from water dissociation
We succeeded in detecting molecular hydrogen derived from water dissociation (WD) using a gastight two-compartment cell (2CC) separated by a bipolar...
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Investigating the Interaction Between Ice-Binding Proteins and Ice Surfaces Using Microfluidic Devices and Cold Stages
Ice-binding proteins (IBPs) protect organisms living in sub-freezing conditions by inhibiting ice growth in fish and insects, limiting ice...
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Super-Resolution Imaging of Tau Proteins in Isolated and Immobilized Brain Synaptosomes
Tau protein has important physiological functions at both presynaptic and postsynaptic terminals. Pathological tau species are also associated with...
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Detection of Glymphatic Outflow of Tau from Brain to Cerebrospinal Fluid in Mice
Glymphatic system denotes a brain-wide pathway that eliminates extracellular solutes from brain. It is driven by the flow of brain interstitial fluid...
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Simple and portable on-site system for nucleic acid-based detection of Clostridium difficile in stool samples using two columns containing microbeads and loop-mediated isothermal amplification
It is challenging to employ nucleic acid-based diagnostics for the in situ detection of Clostridium difficile from complex fecal samples because...
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Isothermal Titration Calorimetry for Quantification of Protein–Carbohydrate Interactions
Isothermal titration calorimetry allows the determination of thermodynamic parameters for the interaction between a protein and mono- or...
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Microfluidics-Enabled Isolation and Single-Cell Analysis of Circulating Tumor Cells
Microfluidic platforms enable the enrichment and analysis of circulating tumor cells (CTCs), a potential biomarker for cancer diagnosis, prognosis,...
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Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry
Isothermal titration calorimetry (ITC) is a technique where the heat given off, or absorbed, during a binding event is measured and used to determine...
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A porous graphene sorbent coated with titanium(IV)-functionalized polydopamine for selective lab-in-syringe extraction of phosphoproteins and phosphopeptides
A novel polydopamine coated three-dimensional porous graphene aerogel sorbent carrying immobilized titanium(IV) ions (denoted as Ti 4+ @PDA@GA) was...
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Structure-Guided and Phage-Assisted Evolution of Therapeutic Antibodies to Reverse On-Target Point Mutation-Mediated Resistance
Resistance to therapeutic antibodies caused by on-target point mutations is a major obstacle in anticancer therapy, creating an “unmet clinical...
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Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension
Most pharmaceutical biotechnology companies use stirred-tank bioreactors (STR) for recombinant protein manufacturing. These bioreactors are used at a...
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Isolation of Extracellular Vesicles from Cell Culture and Blood Through Nano-Targeted DLD Microfluidic Device
Extracellular vesicles (EVs) are secreted by cells and found in biological fluids such as blood, with concentration correlated with oncogenic...
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Mouse Models of Tumor Bone Metastasis and Invasion for Studying CCN Proteins
Bone metastasis and bone destruction are common occurrences in human malignancies, including breast, prostate, and lung cancer, and are associated...
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Ice Shell Purification of Ice-Active Compounds
Ice fractionation allows for the selective purification of ice-active compounds from aqueous solutions. A variety of approaches have been developed...