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Recombinant Protein Expression in Mammalian Cells

Methods and Protocols

  • Book
  • © 2024
  • Latest edition

Overview

Editors:
  1. David L. Hacker

    1. Ecole Polytechnique Fédérale de Lausan, Prangins, Switzerland

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2810)

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About this book

This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell surface protein ectodomain expression and for identifying host genes involved in protein production. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols, Second Edition serves as an ideal guide for researchers investigatingprotein structure and function and accelerating the discovery of new therapeutic proteins.

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. PEI-Mediated Transient Gene Expression in CHO Cells

    • Yashas Rajendra
    Pages 1-10

  3. Transient Co-expression of Membrane Protein Complexes in Mammalian Cells

    • Tânia F. Custódio, Gabriela Guédez, Christian Löw
    Pages 11-28

  4. The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production

    • Julia Flock, Yexin **e, Régis Lemaitre, Karine Lapouge, Kim Remans
    Pages 29-53

  5. Production and Purification of Adeno-Associated Viral Vectors (AAVs) Using Orbitally Shaken HEK293 Cells

    • Jean-Philippe Gaudry, Aline Aebi, Pamela Valdés, Bernard L. Schneider
    Pages 55-74

  6. Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor

    • Jenny Gunnarsson, David I. Fisher, Robert G. Roth
    Pages 75-83

  7. Small-Scale Cultivation and Transfection of ExpiCHO and HEK293E Cells in Single-Use Orbitally Shaken Bioreactors

    • David L. Hacker, Laurence Durrer, Soraya Quinche, Kelvin Lau, Florence Pojer
    Pages 85-98

  8. Recombinant Protein Production from Stable CHO Cell Pools

    • Laurence Delafosse, Simon Lord-Dufour, Alex Pelletier, Sylvie Perret, Alina Burlacu, Manon Ouimet et al.
    Pages 99-121

  9. A Versatile Method for Inducible Protein Production in 293 Cells Using the PiggyBac Transposon System

    • Iacovos P. Michael
    Pages 123-135

  10. Recombinant CHO Cell Pool Generation Using PiggyBac Transposon System

    • Sowmya Balasubramanian
    Pages 137-146

  11. Stable Expression by Lentiviral Transduction of Cells

    • Natascha Gödecke, Hansjörg Hauser, Dagmar Wirth
    Pages 147-159

  12. Expression of Multispecific Antibodies

    • Megane Peltret, Angelique Schmid, Lionel Duarte, Romain Mette, Roberto Giovannini, Martin Bertschinger
    Pages 161-180

  13. Expression of Bispecific Antibodies Using Cellular Display

    • Timothée Brunstein-Laplace, Maria Tsachaki, Megane Peltret, Roberto Giovannini, Martin Bertschinger
    Pages 181-196

  14. Generation of Monoclonal Chinese Hamster Ovary Cell Lines Using DISPENCELL-S3

    • Audrey Berger, Marie Vingerhoets, Arnaud Gelb, Georges Muller, David Bonzon
    Pages 197-210

  15. Enhancing Cell Line Stability by CRISPR/Cas9-Mediated Site-Specific Integration Based on Histone Modifications

    • Oliver Hertel, Anne Neuss
    Pages 211-233

  16. Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension

    • Dominique Monteil, Jeffrey Kuan
    Pages 235-247

  17. Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells

    • Qiong Wang, Lateef Aliyu, Cheng-Yu Chung, Julian N. Rosenberg, Geng Yu, Michael J. Betenbaugh
    Pages 249-271

  18. Development of Responsive Promoters and their Utilization for Stable CHO Sensor Cell Lines

    • Nikolas Zeh, Kerstin Otte
    Pages 273-284

  19. CRISPR Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells

    • Kevin Kellner, Nga T. Lao, Niall Barron
    Pages 285-300

  20. High-Throughput Protein Expression Screening of Cell-Surface Protein Ectodomains

    • Anita Ghosh, Chang Yang, Kenneth Lloyd, Rob Meijers
    Pages 301-316
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Editors and Affiliations

  • Ecole Polytechnique Fédérale de Lausan, Prangins, Switzerland

    David L. Hacker

Bibliographic Information

  • Book Title: Recombinant Protein Expression in Mammalian Cells

  • Book Subtitle: Methods and Protocols

  • Editors: David L. Hacker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3878-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3877-4Published: 27 June 2024

  • Softcover ISBN: 978-1-0716-3880-4Due: 31 July 2024

  • eBook ISBN: 978-1-0716-3878-1Published: 26 June 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 357

  • Number of Illustrations: 12 b/w illustrations, 57 illustrations in colour

  • Topics: Chemistry/Food Science, general, Biological Techniques

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