Gas Chromatography

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Nielsen's Food Analysis Laboratory Manual

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Abstract

Gas chromatography (GC) has many applications in the analysis of food products. A common GC application is fatty acid profile analysis, to meet the nutrition labeling requirements for total fat and saturated fat. This laboratory exercise involves solvent fat extraction, followed by saponification and then methylation with boron trifluoride to form fatty acid methyl esters. The sample is then subjected to GC analysis, with quantification and identification made possible using a reference standard.

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Resource Materials

  • Chen B, Ellefson WC (2024) Fat analysis. Ch. 17. In: Ismail BP, Nielsen SS (eds) Nielsen’s food analysis, 6th edn. Springer, New York

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  • Pike OA (2024) Fat characterization. Ch. 23. In: Ismail BP, Nielsen SS (eds) Nielsen’s food analysis, 6th edn. Springer, New York

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  • Reineccius GA, Qian MC (2024) Gas chromatography. Ch. 14. In: Ismail BP, Nielsen SS (eds) Nielsen’s food analysis, 6th edn. Springer, New York

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Acknowledgments

The authors of this chapter thank Michael C. Qian (Department of Food Science and Technology, Oregon State University, Corvallis, OR, USA) for contributing material for this chapter when it was originally developed.

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Correspondence to B. Pam Ismail .

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© 2024 The Author(s), under exclusive license to Springer Nature Switzerland AG

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Ismail, B.P., Nielsen, S.S. (2024). Gas Chromatography. In: Ismail, B.P., Nielsen, S.S. (eds) Nielsen's Food Analysis Laboratory Manual. Food Science Text Series. Springer, Cham. https://doi.org/10.1007/978-3-031-44970-3_9

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