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Carbohydrate-Protein Interactions

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. D. Wade Abbott

    1. Agriculture and Agri-Food Canada, Lethbridge Research and Development Center, Lethbridge, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Wesley F. Zandberg

    1. Department of Biochemistry, The University of British Columbia, Kelowna, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2657)

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About this book

This second edition provides new and updated tools for studying protein-carbohydrate interactions ranging from traditional biochemical methods to state-of-the-art techniques. This book focuses on four different research themes detailing methods for screening and quantifying CAZyme activity, investigating the interactions between proteins, carbohydrate ligands, methods for the visualization of carbohydrates, protein-carbohydrate complexes, structural and “omic” approaches for studying systems of CAZymes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Carbohydrate- Protein Interactions: Methods and Protocols, Second Edition aims to be  comprehensive guide for researchers inthe field.

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Measurement of Carbohydrate Modification

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases

      • Gregory Arnal, Mohamed A. Attia, Jathavan Asohan, Zhenhuan Lei, Benedikt Golisch, Harry Brumer
      Pages 3-14

    3. Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay

      • He Li, Lauren S. McKee
      Pages 15-25

    4. Analyzing Activities of Lytic Polysaccharide Monooxygenases by Liquid Chromatography and Mass Spectrometry

      • Bjørge Westereng, Magnus Ø. Arntzen, Heidi Østby, Jane Wittrup Agger, Gustav Vaaje-Kolstad, Vincent G. H. Eijsink
      Pages 27-51

    5. Carbohydrate Depolymerization by Intricate Cellulosomal Systems

      • Sarah Moraïs, Johanna Stern, Lior Artzi, Carlos M. G. A. Fontes, Edward A. Bayer, Itzhak Mizrahi
      Pages 53-77

    6. Biomechanical Weakening of Paper and Plant Cell Walls by Bacterial Expansins

      • Daniel J. Cosgrove, Nathan K. Hepler, Edward R. Wagner, Daniel M. Durachko
      Pages 79-88

  3. Quantitation of Carbohydrate Binding

    1. Front Matter

      Pages 89-89
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    2. Affinity Electrophoresis for Analysis of Catalytic Module–Carbohydrate Interactions

      • Darrell W. Cockburn, Casper Wilkens, Birte Svensson
      Pages 91-101

    3. Quantifying CBM–Carbohydrate Interactions Using Microscale Thermophoresis

      • Haiyang Wu, Cédric Y. Montanier, Claire Dumon
      Pages 103-114

    4. Qualitative and Quantitative Characterization of Protein–Carbohydrate Interactions by NMR Spectroscopy

      • Julie M. Grondin, David N. Langelaan, Steven P. Smith
      Pages 115-128

    5. Isothermal Titration Calorimetry for Quantification of Protein–Carbohydrate Interactions

      • Haley A. Brown, Nicole M. Koropatkin
      Pages 129-140

    6. Surface Plasmon Resonance Analysis for Quantifying Protein–Carbohydrate Interactions

      • Marie Sofie Møller, Darrell W. Cockburn, Casper Wilkens
      Pages 141-150

    7. Bioinspired Polymer Assemblies of Plant Cell Walls for Measuring Protein–Carbohydrate Interactions by FRAP

      • Berangère Lebas, Gabriel Paës
      Pages 151-162

    8. CBMs as Probes to Explore Plant Cell Wall Heterogeneity Using Immunocytochemistry

      • Louise Badruna, Vincent Burlat, Cédric Y. Montanier
      Pages 163-179

    9. Flow Cytometry-Based Detection of Siglec Ligands

      • Edward N. Schmidt, Jaesoo Jung, Matthew S. Macauley
      Pages 181-193

  4. Purification and Visualization of Carbohydrates

    1. Front Matter

      Pages 195-195
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    2. Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material

      • Mackenzie Melvin, Noah Fancy, Darrek Kniffen, Kirk Bergstrom
      Pages 197-205

    3. Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transform Mid-Infrared Spectroscopy

      • Ajay Badhan, Yuxi Wang, Tim A. McAllister
      Pages 207-213

    4. Separation and Visualization of Glycans by Fluorophore-Assisted Carbohydrate Electrophoresis

      • Mélissa Robb, Joanne K. Hobbs, Alisdair B. Boraston
      Pages 215-222
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Editors and Affiliations

  • Agriculture and Agri-Food Canada, Lethbridge Research and Development Center, Lethbridge, Canada

    D. Wade Abbott

  • Department of Biochemistry, The University of British Columbia, Kelowna, Canada

    Wesley F. Zandberg

Bibliographic Information

  • Book Title: Carbohydrate-Protein Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: D. Wade Abbott, Wesley F. Zandberg

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3151-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3150-8Published: 07 May 2023

  • Softcover ISBN: 978-1-0716-3153-9Published: 07 May 2023

  • eBook ISBN: 978-1-0716-3151-5Published: 06 May 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 318

  • Number of Illustrations: 23 b/w illustrations, 37 illustrations in colour

  • Topics: Chemistry/Food Science, general, Biochemistry, general

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