Abstract
Microfluidic platforms enable the enrichment and analysis of circulating tumor cells (CTCs), a potential biomarker for cancer diagnosis, prognosis, and theragnosis. Combined with immunocytochemistry/immunofluorescence (ICC/IF) assays for CTCs, microfluidics-enabled detection presents a unique opportunity to study tumor heterogeneity and predict treatment response, both of which can help cancer drug development. In this chapter, we detail the protocols and methods employed to fabricate and use a microfluidic device for the enrichment, detection, and analysis of single CTCs from the blood samples of sarcoma patients.
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Acknowledgments
The authors would like to thank Beate Greer for assisting with the clinical aspect of this work and the patients for generously providing the samples. M.N.L. would like to acknowledge the support from the National Science Foundation Graduate Research Fellowship Program (DGE-1842473). This work has been supported in part by the National Institutes of Health (R01CA238387), the Florida Department of Health (23L05), and the University of Florida, USA.
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Le, MC.N., Smith, K.A., Alipanah, M., Chen, K., Lagmay, J.P., Fan, Z.H. (2023). Microfluidics-Enabled Isolation and Single-Cell Analysis of Circulating Tumor Cells. In: Li, P.C., Wu, A.R. (eds) Single-Cell Assays. Methods in Molecular Biology, vol 2689. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3323-6_7
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DOI: https://doi.org/10.1007/978-1-0716-3323-6_7
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