12 Result(s)
within
Vladimir Sidorov
English
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Article
Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts
Application of new genome editing technologies for plant improvement depends on efficient delivery of editing components into plant cells. For editing of canola, which is an important oil crop, CRISPR/Cas9 and Ag...
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Article
Open AccessDemonstration of targeted crossovers in hybrid maize using CRISPR technology
Naturally occurring chromosomal crossovers (CO) during meiosis are a key driver of genetic diversity. The ability to target CO at specific allelic loci in hybrid plants would provide an advantage to the plant ...
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Article
“Cell grafting”: a new approach for transferring cytoplasmic or nuclear genome between plants
A new method based on mixing and wounding of callus tissue was used to transfer plastid or nuclear DNA between cells.
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Protocol
Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus
Transformation with Agrobacterium tumefaciens is the preferred method for delivery of transgenes into a wide range of plant species including maize. Optimized protocols for the Agrobacterium-mediated transformati...
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Article
Physical Characteristics of Kazan Minor Showers as Determined by Correlations with the Arecibo UHF Radar
In the northern hemisphere, the month of February is characterized by a lack of major meteor shower activity yet a number of weak minor showers are present as seen by the Kazan radar. Using the Feller transfor...
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Chapter
Physical Characteristics of Kazan Minor Showers as Determined by Correlations with the Arecibo UHF Radar
In the northern hemisphere, the month of February is characterized by a lack of major meteor shower activity yet a number of weak minor showers are present as seen by the Kazan radar. Using the Feller transfor...
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Article
Agrobacterium-mediated transformation of seedling-derived maize callus
Efficient production of seedling-derived Type I callus was demonstrated for several corn genotypes including commercial inbred lines. Seeds were germinated on MS-based medium containing 10 mg l−1 picloram and 3 m...
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Chapter and Conference Paper
Industrial Application of External Black-Box Functions in Constraint Programming Solver
We examine the problem of interaction between the interval constraint programming solver and external computation algorithms that are considered by the solver as Black Boxes. Some techniques are used in order ...
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Chapter and Conference Paper
Constraint Programming Techniques for Solving Problems on Graphs
In this paper we examine a technology for solving problems on graphs in the constraint programming framework called Subdefinite Models. We describe in brief the mechanism of constraint propagation underlying it. ...
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Chapter and Conference Paper
Problem solving in the object-oriented technological environment NeMo+
We study the mechanism of subdefinite models and the object-oriented technological environment NeMo+ for constructing solvers based on subdefinite models. The paper presents the main features of NeMo+ and shows i...
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Article
Complementation in somatic hybrids indicates four types of nitrate reductase deficient lines in Nicotiana plumbaginifolia
Allelism of nine nitrate reductase deficient (NR−) Nicotiana plumbaginifolia cell lines was tested by complementation after protoplast fusion. Complementation was recognized by the appearance of somatic hybrid co...
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Article
Isoleucine-requiring Nicotiana plant deficient in threonine deaminase
Well characterized auxotrophic mutants would greatly assist the genetic manipulation of flowering plants, but such mutants are rare1–3. Moreover, auxotrophic mutants isolated at the plant level4–8 and some of tho...
