Skip to main content

4 Result(s)

within Larry Gilbertson Remove this filter

and

  1. No Access

    Article

    Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts

    Application of new genome editing technologies for plant improvement depends on efficient delivery of editing components into plant cells. For editing of canola, which is an important oil crop, CRISPR/Cas9 and Ag...

    Vladimir Sidorov, Dafu Wang, Ervin D. Nagy… in In Vitro Cellular & Developmental Biology … (2022)

  2. No Access

    Article

    Agrobacterium-mediated transformation of seedling-derived maize callus

    Efficient production of seedling-derived Type I callus was demonstrated for several corn genotypes including commercial inbred lines. Seeds were germinated on MS-based medium containing 10 mg l−1 picloram and 3 m...

    Vladimir Sidorov, Larry Gilbertson, Prince Addae, David Duncan in Plant Cell Reports (2006)

  3. No Access

    Article

    Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors

    By introducing additional T-DNA borders into a binary plasmid used in Agrobacterium-mediated plant transformation, previous studies have demonstrated that the marker gene and the gene of interest (GOI) can be ...

    Shihshieh Huang, Larry Gilbertson, Tom Adams, Kathleen Malloy… in Transgenic Research (2004)

  4. No Access

    Chapter

    Cre/lox Mediated Marker Gene Excision in Transgenic Crop Plants

    Selectable marker genes are used in most plant transformation processes. Frequently, however, the selectable marker gene does not confer useful traits on the transgenic plant, and there are a number of advanta...

    Larry Gilbertson, Waly Dioh, Prince Addae… in Plant Biotechnology 2002 and Beyond (2003)