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Article
Open AccessTransposase-assisted target-site integration for efficient plant genome engineering
The current technologies to place new DNA into specific locations in plant genomes are low frequency and error-prone, and this inefficiency hampers genome-editing approaches to develop improved crops1,2. Often co...
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Article
Cre-mediated autoexcision of selectable marker genes in soybean, cotton, canola and maize transgenic plants
Efficient selectable marker gene autoexcision in transgenic plants of soybean, cotton, canola, and maize is achieved by effective Cre recombinase expression.
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Article
Open AccessAn improved calibration and uncertainty analysis approach using a multicriteria sequential algorithm for hydrological modeling
Hydrological models are widely used as simplified, conceptual, mathematical representatives for water resource management. The performance of hydrological modeling is usually challenged by model calibration an...
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Article
Sulfate removal using colloid-enhanced ultrafiltration: performance evaluation and adsorption studies
Colloid-enhanced ultrafiltration (CEUF), i.e., micellar-enhanced ultrafiltration (MEUF) and polymer-enhanced ultrafiltration (PEUF), was investigated to remove sulfate ions from aqueous solution in batch exper...
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Article
“Cell grafting”: a new approach for transferring cytoplasmic or nuclear genome between plants
A new method based on mixing and wounding of callus tissue was used to transfer plastid or nuclear DNA between cells.
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Article
Constitutive expression of the tzs gene from Agrobacterium tumefaciens virG mutant strains is responsible for improved transgenic plant regeneration in cotton meristem transformation
virG mutant strains of a nopaline type of Agrobacterium tumefaciens increase the transformation frequency in cotton meristem transformation...
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Article
High throughput Agrobacterium tumefaciens-mediated germline transformation of mechanically isolated meristem explants of cotton (Gossypium hirsutum L.)
Agrobacterium tumefaciens mediates high frequency of germline transformation of cotton meristem explants. The meristem transformation system we developed is rapid, high throug...
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Article
Robust adaptive repetitive learning control for a class of time-varying nonlinear systems with unknown control direction
A robust adaptive repetitive learning control method is proposed for a class of time-varying nonlinear systems. Nussbaum-gain method is incorporated into the control design to counteract the lack of a priori k...
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Article
Enhanced production of single copy backbone-free transgenic plants in multiple crop species using binary vectors with a pRi replication origin in Agrobacterium tumefaciens
Single transgene copy, vector backbone-free transgenic crop plants are highly desired for functional genomics and many biotechnological applications. We demonstrate that binary vectors that use a replication o...
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Article
Plant development inhibitory genes in binary vector backbone improve quality event efficiency in soybean transformation
Conventional Agrobacterium-mediated plant transformation often produces a significant frequency of transgenic events containing vector backbone sequence, which is generally undesirable for biotechnology applicati...
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Article
Factors influencing Agrobacterium-mediated transformation of monocotyledonous species
Since the success of Agrobacterium-mediated transformation of rice in the early 1990s, significant advances in Agrobacterium-mediated transformation of monocotyledonous plant species have been achieved. Transgeni...
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Article
Effective selection and regeneration of transgenic rice plants with mannose as selective agent
A new method for the selection of transgenic rice plants without the use of antibiotics or herbicides has been developed. The phosphomannose isomerase (PMI) gene from Escherichia coli has been cloned and consitut...
