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Article
Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts
Application of new genome editing technologies for plant improvement depends on efficient delivery of editing components into plant cells. For editing of canola, which is an important oil crop, CRISPR/Cas9 and Ag...
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Article
Open AccessDemonstration of targeted crossovers in hybrid maize using CRISPR technology
Naturally occurring chromosomal crossovers (CO) during meiosis are a key driver of genetic diversity. The ability to target CO at specific allelic loci in hybrid plants would provide an advantage to the plant ...
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Article
“Cell grafting”: a new approach for transferring cytoplasmic or nuclear genome between plants
A new method based on mixing and wounding of callus tissue was used to transfer plastid or nuclear DNA between cells.
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Chapter
Political Religion
The concept of “political religion” has its roots in the European Enlightenment of the eighteenth century, when the growth of secularism and the separation of political legitimacy from divine right led to new ...
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Chapter
Transient Expression and Stable Transformation of Maize Using Microprojectiles
High-velocity microprojectiles provide a direct means of introducing DNA into plant cells (Klein et al., 1987, 1988a, 1988b; Wang et al., 1988). In principle, this method could be used to introduce DNA into any a...
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Article
Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants
We obtained transgenic maize plants by using high-velocity microprojectiles to transfer genes into embryogenic cells. Two selectable genes were used to confer resistance to either chlorsulfuron or phosphinothr...
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Medical miscellany
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Article
Medical miscellany