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  1. No Access

    Article

    Plant regeneration from cell suspension-derived protoplasts of Nicotiana africana

    Plants have been regenerated from Nicotiana africana Merxm. protoplasts isolated from cell suspensions. Two different sequences of media were assayed, one usually used to regenerate tobacco mesophyll protoplas...

    Lenuţa Rákosy-Tican, László Menczel in Plant Cell, Tissue and Organ Culture (1998)

  2. No Access

    Article

    Somatic embryogenesis and plant regeneration from hypocotyl protoplasts of sunflower (Helianthus annum L.)

    A sunflower genotype (Helianthus annuus L. cv. Florom-328) able to regenerate plants from in vitro cultures was identified by screening hybrids and inbred lines. Protoplasts of this genotype were isolated from da...

    Sergei Krasnyanski, László Menczel in Plant Cell Reports (1993)

  3. No Access

    Article

    Plant regeneration from callus and protoplast cultures of Helianthus giganteus L.

    Methods of plant regeneration from callus and protoplasts of Helianthus giganteus L. are described. Embryogenic callus was obtained from leaf explants and plants were regenerated from these calli on MS media with...

    Sergei Krasnyanski, Zsolt Polgár, Gizella Németh, László Menczel in Plant Cell Reports (1992)

  4. No Access

    Article

    Transfer of cytoplasmic male sterility by selection for streptomycin resistance after protoplast fusion in Nicotiana

    Cytoplasmic male sterility (CMS) in Nicotiana is located on mitochondrial DNA (mtDNA) on which no selectable mutation has been isolated. The possibility of co-transfer of CMS and a selectable chloroplast trait, s...

    László Menczel, Ferenc Nagy, Gabriella Lázár… in Molecular and General Genetics MGG (1983)

  5. No Access

    Article

    Isoleucine-requiring Nicotiana plant deficient in threonine deaminase

    Well characterized auxotrophic mutants would greatly assist the genetic manipulation of flowering plants, but such mutants are rare1–3. Moreover, auxotrophic mutants isolated at the plant level4–8 and some of tho...

    Vladimir Sidorov, László Menczel, Pál Maliga in Nature (1981)

  6. No Access

    Article

    Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate treated protoplasts

    Protoplasts of a light sensitive plastome mutant of Nicotiana tabacum (2 n=48) were irradiated and fused with iodoacetate-treated Nicotiana plumbaginifolia (2 n=20) protoplasts. Treated parental protoplasts were ...

    Vladimir A. Sidorov, László Menczel, Ferenc Nagy, Pál Maliga in Planta (1981)

  7. No Access

    Article

    The use of cytoplasmic streptomycin resistance: Chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris, and Isolation of their somatic hybrids

    Leaf protoplasts of Nicotiana tabacum SR1 (2n=4x=48) treated with iodoacetate (10 mM; 25 C; 30 min) and consequently unable to divide, and untreated leaf protoplasts of Nicotiana sylvestris (2n=2x=24) were fused ...

    Péter Medgyesy, László Menczel, Pál Maliga in Molecular and General Genetics MGG (1980)

  8. No Access

    Article

    Restoration of morphogenic potential in Nicotiana by somatic hybridisation

    KR103 is a kanamycin resistant cell line of Nicotiana sylvestris, deficient in inducible shoot redifferentiation. The existence of a genetically unaltered inducibility factor in KR103 was tested by fusing protopl...

    Pál Maliga, Gabriella Lázár, Ferenc Joó… in Molecular and General Genetics MGG (1977)