Abstract
Well characterized auxotrophic mutants would greatly assist the genetic manipulation of flowering plants, but such mutants are rare1–3. Moreover, auxotrophic mutants isolated at the plant level4–8 and some of those isolated in cell cultures9 are leaky. The isolation of the first non-leaky auxotrophic mutant, requiring a source of nitrogen in reduced form, was possible by direct selection for chlorate resistance of this rare mutant type10. A different approach, the individual testing of colonies grown from mutagenized haploid cells of Datura11 and Hyoscyam us12, has resulted in cell lines with absolute nutritional requirement. We report here the isolation of an isoleucine-requiring line from haploid cell cultures of Nicotiana plumbaginifolia and the regeneration of diploid plants. These plants have no detectable activity of L-threonine deaminase (EC 4.2.1.16), the first enzyme in isoleucine biosynthesis.
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References
Widholm, J. M. in Plant Tissue Culture and its Biotechnological Application (eds Barz, W., Reinhard, E. & Zenk, M. H.) 112–122 (Springer, Berlin, 1977).
Schieder, O. in Frontiers of Plant Tissue Culture (ed. Thorpe, T. A.) 393–401 (University of Calgary Offset Printing Service, 1978).
Maliga, P. Int. Rev. Cytol. Suppl. 11A, 225–250 (1980).
Rédei, G. P. A. Rev. Genet. 9, 111–127 (1975).
Oostindier-Braaksma, F. J. & Feenstra, W. J. Mutat. Res. 19, 175–185 (1973).
Gavazzi, G., Nava-Racchi, M. & Tonelli, C. Theor. appl. Genet. 46, 339–345 (1975).
Warner, R. L., Lin, C. J. & Kleinhofs, A. Nature 269, 406–407 (1977).
Feenstra, W. J. & Jacobson, E. Theor. appl. Genet. 58, 39–42 (1980).
Carlson, P. S. Science 168, 487–489 (1970).
Müller, A. & Grafe, R. Molec. gen. Genet. 161, 67–76 (1978).
Savage, A. D., King, J. & Gamborg, O. L. Pl. Sci. Lett. 16, 367–376 (1979).
Gebhard, C., Schnebli, V. & King, P. Planta (in the press).
Holliday, R. Nature 178, 987 (1956).
Bayliss, M. W. Int. Rev. Cytol. Suppl. 11 A, 113–144 (1980).
Umbarger, H. E. A. Rev. Biochem. 47, 533–606 (1978).
Miflin, B. J. & Lea, P. J. A. Rev. Pl. Physiol. 28, 299–329 (1977).
Dougall, D. K. Phytochemistry 9, 959–964 (1970).
Sharma, R. K. & Mazumder, R. J. biol. Chem. 245, 3008–3014 (1970).
Umbarger, H. E., McElroy, W. D. & Glass, H. B. Amino Acid Metabolism (Johns Hopkins University Press, Baltimore, 1956).
Miflin, B. J. & Cave, P. R. J. exp. Botany 23, 511–516 (1972).
Medgyesy, P., Menczel, L. & Maliga, P. Molec. gen. Genet. 179, 693–698 (1980).
Sidorov, V., Menczel, L., Nagy, F. & Maliga, P. Planta 152, 341–345 (1981).
Nagy, J. I. & Maliga, P. Z. Pflanzenphysiol. 78, 453–455 (1976).
Linsmaier, E. M. & Skoog, F. Physiologia Pl. 18, 100–127 (1965).
Friedemann, T. E. & Haugen, E. G. J. biol. Chem. 147, 415–443 (1943).
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Sidorov, V., Menczel, L. & Maliga, P. Isoleucine-requiring Nicotiana plant deficient in threonine deaminase. Nature 294, 87–88 (1981). https://doi.org/10.1038/294087a0
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DOI: https://doi.org/10.1038/294087a0
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