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  1. No Access

    Article

    Triazine-resistant Nicotiana mutants from photomixotrophic cell cultures

    Triazine-resistant mutants have been isolated in photomixotrophic cell cultures of Nicotiana plumbaginifolia. Triazine herbicides inhibit photosynthesis and cause extensive photodestruction of chloroplasts (bleac...

    Agnes Csépl'ó, Péter Medgyesy, Éva Hideg… in Molecular and General Genetics MGG (1985)

  2. No Access

    Article

    Interspecific protoplast fusion to rescue a cytoplasmic lincomycin resistance mutation into fertile Nicotiana plumbaginifolia plants

    A lincomycin-resistant cell line, LR105, was isolated in a mutagenized (0.1 mM N-ethyl-N-nitrosourea) callus culture initiated from a haploid Nicotiana sylvestris plant. The regenerated plants had an abnormal mor...

    Ágnes Cséplő, Ferenc Nagy, Pál Maliga in Molecular and General Genetics MGG (1984)

  3. No Access

    Article

    Large scale isolation of maternally inherited lincomycin resistance mutations, in diploid Nicotiana plumbaginifolia protoplast cultures

    Lincomycin-resistant clones were isolated in diploid protoplast cultures of Nicotiana plumbaginifolia. Selection of the resistant clones was based on the ability of resistant calli to green in the presence of the...

    Ágnes Cséplő, Pál Maliga in Molecular and General Genetics MGG (1984)

  4. No Access

    Article

    Transfer of cytoplasmic male sterility by selection for streptomycin resistance after protoplast fusion in Nicotiana

    Cytoplasmic male sterility (CMS) in Nicotiana is located on mitochondrial DNA (mtDNA) on which no selectable mutation has been isolated. The possibility of co-transfer of CMS and a selectable chloroplast trait, s...

    László Menczel, Ferenc Nagy, Gabriella Lázár… in Molecular and General Genetics MGG (1983)

  5. No Access

    Article

    Lincomycin resistance, a new type of maternally inherited mutation in Nicotiana plumbaginifolia

    Lincomycin resistant cell lines were screened in monoploid (X = 10 chromosomes) protoplast cultures of Nicotiana plumbaginifolia. Lincomycin is an inhibitor of protein synthesis on plastid ribosomes and normally ...

    Ágnes Cséplő, Pál Maliga in Current Genetics (1982)

  6. No Access

    Article

    Nitrate reductase deficient cell lines from haploid protoplast cultures ofNicotiana plumbaginifolia

    Nitrate reductase deficient (NR-) cell lines were selected indirectly by their resistance to 40 mM chlorate in protoplast cultures of haploidNicotiana plumbaginifolia. Frequency of the chlorate resistant clones w...

    László Márton, Trinh Manh Dung, Ralf R. Mendel… in Molecular and General Genetics MGG (1982)

  7. No Access

    Article

    Complementation in somatic hybrids indicates four types of nitrate reductase deficient lines in Nicotiana plumbaginifolia

    Allelism of nine nitrate reductase deficient (NR−) Nicotiana plumbaginifolia cell lines was tested by complementation after protoplast fusion. Complementation was recognized by the appearance of somatic hybrid co...

    László Márton, Vladimir Sidorov, Grazia Biasini… in Molecular and General Genetics MGG (1982)

  8. No Access

    Article

    Cytoplast-protoplast fusion for interspecific chloroplast transfer in Nicotiana

    Protoplasts of Nicotiana tabacum (SR1), carrying a maternally-inherited streptomycin resistance mutation, were enucleated by centrifugation through a Percoll gradient. The resulting cytoplasts containing resistan...

    Pál Maliga, Horst Lörz, Gabriella Lázár, Ferenc Nagy in Molecular and General Genetics MGG (1982)

  9. No Access

    Article

    Isoleucine-requiring Nicotiana plant deficient in threonine deaminase

    Well characterized auxotrophic mutants would greatly assist the genetic manipulation of flowering plants, but such mutants are rare1–3. Moreover, auxotrophic mutants isolated at the plant level4–8 and some of tho...

    Vladimir Sidorov, László Menczel, Pál Maliga in Nature (1981)

  10. No Access

    Article

    Extensive rearrangements in the mitochondrial DNA in somatic hybrids of Nicotiana tabacum and Nicotiana knightiana

    Mitochondrial DNA (mtDNA) was studied in the leaves of Nicotiana tabacum+Nicotiana knightiana somatic hybrids previously described. Restriction patterns generated by the SalI and BamHI restriction endonucleases w...

    Ferene Nagy, István Török, Pál Maliga in Molecular and General Genetics MGG (1981)

  11. No Access

    Article

    Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate treated protoplasts

    Protoplasts of a light sensitive plastome mutant of Nicotiana tabacum (2 n=48) were irradiated and fused with iodoacetate-treated Nicotiana plumbaginifolia (2 n=20) protoplasts. Treated parental protoplasts were ...

    Vladimir A. Sidorov, László Menczel, Ferenc Nagy, Pál Maliga in Planta (1981)

  12. No Access

    Article

    The use of cytoplasmic streptomycin resistance: Chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris, and Isolation of their somatic hybrids

    Leaf protoplasts of Nicotiana tabacum SR1 (2n=4x=48) treated with iodoacetate (10 mM; 25 C; 30 min) and consequently unable to divide, and untreated leaf protoplasts of Nicotiana sylvestris (2n=2x=24) were fused ...

    Péter Medgyesy, László Menczel, Pál Maliga in Molecular and General Genetics MGG (1980)

  13. No Access

    Article

    Genetic instability in somatic hybrids of Nicotiana tabacum and Nicotiana knightiana

    Somatic hybrids of Nicotiana knightiana (2n=2X=24) and an albino mutant of Nicotiana tabacum (2n=4X=48) were selected after polyethylene glycol induced protoplast fusion. Three lines were selected on the basis of...

    Pál Maliga, Zsuzsa R. Kiss, Anna H. Nagy… in Molecular and General Genetics MGG (1978)

  14. No Access

    Article

    Restoration of morphogenic potential in Nicotiana by somatic hybridisation

    KR103 is a kanamycin resistant cell line of Nicotiana sylvestris, deficient in inducible shoot redifferentiation. The existence of a genetically unaltered inducibility factor in KR103 was tested by fusing protopl...

    Pál Maliga, Gabriella Lázár, Ferenc Joó… in Molecular and General Genetics MGG (1977)

  15. No Access

    Article

    A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin

    Cell lines resistant to 50 μg ml-1 kanamycin sulphate were isolated from cell suspension cultures initiated from a haploid Nicotiana sylvestris plant. One line, KR103, has been studied in detail. Resistance of th...

    Philip J. Dix, Ferenc Joó, Pál Maliga in Molecular and General Genetics MGG (1977)

  16. No Access

    Article

    Transient cycloheximide resistance in a tobacco cell line

    Cycloheximide-resistant cell lines have been recovered from tissue cultures of haploid Nicotiana tabacum at the usual mutant frequency (10-6). One such line, CR 4/6 was studied in detail. Resistance is based on t...

    Pál Maliga, Gabriella Lázár, Zóra Sváb, Ferenc Nagy in Molecular and General Genetics MGG (1976)