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Article
Open AccessShoot tip necrosis of in vitro plant cultures: a reappraisal of possible causes and solutions
Shoot tip necrosis is a physiological condition that negatively impacts the growth and development of in vitro plant shoot cultures across a wide range of species.
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Article
Selenate tolerance and selenium hyperaccumulation in the monocot giant reed (Arundo donax), a biomass crop plant with phytoremediation potential
The response of giant reed (Arundo donax L.) to selenium (Se), added as selenate, was studied. The development, stress response, uptake, translocation, and accumulation of Se were documented in three giant reed e...
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Article
Open AccessEarly post-operative thrombosis of the prosthetic mitral valve in patient with heparin-induced thrombocytopenia
Heparin-induced thrombocytopenia (HIT) is one of the most common immune-mediated adverse drug reactions, with frequencies as high as 2-3% for certain groups of post-cardiac surgery patients. We report on an 50...
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Article
Correction: Corrigendum: Spike timing of dendrite-targeting bistratified cells during hippocampal network oscillations in vivo
Nature Neuroscience 7, 41–47 (2004); published online 23 November 2003; corrected after print 20 June In the version of this article initially published, the dose of urethane anesthesia in the Methods section ...
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Article
Transgenic Spartina alterniflora for phytoremediation
Perennial monoculture forming grasses are very important natural remediators of pollutants. Their genetic improvement is an important task because introduction of key transgenes can dramatically improve their ...
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In vitro Propagation of Wetland Monocots for Phytoremediation
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Über die Frage, ob es möglich ist, mit einem Toten Freundschaft zu schliessen
die Frage meines Vortragstitels ist nicht in dem Maße mysteriös, wie man im ersten Augenblick denken könnte. Es geht nicht etwa um eine Geisterbeschwörung, sondern um ein Problem der Rezeptionsästhetik. Die De...
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Article
Differential mercury volatilization by tobacco organs expressing a modified bacterial merA gene
Mercury pollution is a major environmental problem accompanying industrial activities. Most of the mercury released ends up and retained in the soil as complexes of the toxic ionic mercury (Hg2+), which then can ...
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Article
Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation
When Agrobacterium was used to transform Nicotiana plumbaginifolia protoplasts and Arabidopsis thaliana roots and seedlings, a large number of plants were found in which not only the T-region defined by the bo...
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Article
T-DNA-insert-independent mutations induced in transformed plant cells duringAgrobacterium co-cultivation
Transformation frequencies were determined for 1n, 2n, and 4n Nicotiana plumbaginifolia protoplast cultures inAgrobacterium-mediated gene transfer experiments. An unexpected large drop (50%) in plating efficienci...
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Article
Sustained root culture for generation and vegetative propagation of transgenic Arabidopsis thaliana
Excised roots of wild-type and nitrate-reductase deficient mutant Arabidopsis thaliana (L.) Heynh. can be propagated as sustained root cultures in liquid medium. Culture initiation from a single seedling required...
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Article
Differential manifestation of seed mortality induced by seed-specific expression of the gene for diphtheria toxin A chain in Arabidopsis and tobacco
A pea vicilin promoter-diphtheria toxin A (DTx-A) chain gene fusion was introduced into Arabidopsis and tobacco. The chimeric Dtx-A gene behaves as a dominant, seed-lethal, Mendelian factor, and the segregation r...
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Article
Facile transformation of Arabidopsis
A protocol is described for the simple, rapid and efficient production of transgenic Arabidopsis plants. The procedure was developed using growth regulator regimes that promote adventitious embryogenesis during o...
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Article
Respiratory control over photosynthetic electron transport in chloroplasts of higher-plant cells: evidence for chlororespiration
Flash-induced primary charge separation, detected as electrochromic absorbance change, the operation of the cytochrome b/f complex and the redox state of the plastoquinone pool were measured in leaves, protoplast...
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Article
Intertrubal chloroplast transfer by protoplast fusion between Nicotiana tabacum and Salpiglossis sinuata
Chloroplast tranfer was achieved by protoplast fusion between Nicotiana tobacum (Cestreae, Cestroideae) and Salpiglossis sinuata (Salpiglossideae, Cestroideae) in the family Solanaceae. Isolation of cybrid clones...
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Article
Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia Viv. tissue cultures using the ethylene inhibitor AgNO3
Silver nitrate effectively promoted shoot regeneration in wheat (Triticum aestivum L.) callus cultures derived from immature embryos. This effect could be observed in both weakly and strongly regenerating cultiva...
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Article
Transmission of paternal chloroplasts in Nicotiana
Transmission of paternal chloroplasts was observed in Nicotiana, considered to inherit organelles in a strictly maternal way. Plants carrying streptomycin resistant plastids were used as pollen donors. Cell lines...
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Article
Independent integration and seed-transmission of the TR-DNA of the octopine Ti plasmid pTi Ach5 in Nicotiana plumbaginifolia
After co-cultivation of diploid Nicotiana plumbaginifolia protoplasts with an octopine-type Agrobacterium tumefaciens strain (LBA 4013) putative transformants were selected for hormone-independent growth, and wer...
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Article
Triazine-resistant Nicotiana mutants from photomixotrophic cell cultures
Triazine-resistant mutants have been isolated in photomixotrophic cell cultures of Nicotiana plumbaginifolia. Triazine herbicides inhibit photosynthesis and cause extensive photodestruction of chloroplasts (bleac...
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Article
Nitrate reductase deficient cell lines from haploid protoplast cultures ofNicotiana plumbaginifolia
Nitrate reductase deficient (NR-) cell lines were selected indirectly by their resistance to 40 mM chlorate in protoplast cultures of haploidNicotiana plumbaginifolia. Frequency of the chlorate resistant clones w...
