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Article
Open AccessConstruction of transplantable artificial vascular tissue based on adipose tissue-derived mesenchymal stromal cells by a cell coating and cryopreservation technique
Prevascularized artificial three-dimensional (3D) tissues are effective biomaterials for regenerative medicine. We have previously established a scaffold-free 3D artificial vascular tissue from normal human de...
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Article
Open AccessMechanical activities of self-beating cardiomyocyte aggregates under mechanical compression
Since the discovery of synchronous pulsations in cardiomyocytes (CMs), electrical communication between CMs has been emphasized; however, recent studies suggest the possibility of mechanical communication. Her...
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Article
Open AccessDevelopment of a drug screening system using three-dimensional cardiac tissues containing multiple cell types
We hypothesized that an appropriate ratio of cardiomyocytes, fibroblasts, endothelial cells, and extracellular matrix (ECM) factors would be required for the development of three-dimensional cardiac tissues (3...
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Protocol
Construction of Three-Dimensional Cardiac Tissues Using Layer-by-Layer Method
Myocardial tissues in vivo are complex three-dimensional structures. Significant efforts are currently focused on develo** functionally and structurally similar tissues in vitro to transplant them for regene...
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Article
Open AccessVascularized cardiac tissue construction with orientation by layer-by-layer method and 3D printer
Herein, we report the fabrication of native organ-like three-dimensional (3D) cardiac tissue with an oriented structure and vascular network using a layer-by-layer (LbL), cell accumulation and 3D printing tech...
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Article
Inhibitory effect of carbonyl reductase 1 against peritoneal progression of ovarian cancer: evaluation by ex vivo 3D-human peritoneal model
The current authors previously reported that a carbonyl reductase 1 (CR1) DNA-dendrimer complex could potentially be used in gene therapy for peritoneal metastasis of ovarian cancer. The aims of the current st...
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Article
Open AccessA novel strategy to engineer pre-vascularized 3-dimensional skin substitutes to achieve efficient, functional engraftment
Autologous split-thickness skin grafts are the preferred treatment for excised burn wounds, but donor sites for autografting are often limited in patients with extensive burns. A number of alternative treatmen...
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Chapter
Fabrication Methods of Sustainable Hydrogels
The interest received on hydrogels probably reflects one of the greatest challenges for the two last decades. Able to hold and release solvents and builds, these three-dimensional polymeric structures work as ...
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Article
Development of analytical methods for evaluating the quality of dissociated and associated amphiphilic poly(γ-glutamic acid) nanoparticles
A quantitative method of analyzing nanoparticles (NPs) for drug delivery is urgently required by researchers and industry. Therefore, we developed new quantitative analytical methods for biodegradable and amph...
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Article
A novel comb-shaped polymethacrylate-based copolymers with immobilized 2,4-dihydroxybenzaldehyde for antifungal activity
Poly(methacrylic acid-co-2-aminoethyl methacrylate) (MAA-AMA) was simply synthesized by free radical polymerization. Immobilization of 2,4-dihydroxybenzaldehyde on MAA-AMA was done via Schiff base formation to ob...
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Article
Open AccessEngraftment and morphological development of vascularized human iPS cell-derived 3D-cardiomyocyte tissue after xenotransplantation
One of the major challenges in cell-based cardiac regenerative medicine is the in vitro construction of three-dimensional (3D) tissues consisting of induced pluripotent stem cell-derived cardiomyocyte (iPSC-CM) a...
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Article
Pentaerythritol particles covered by layer-by-layer self assembled thin films with stereocomplex of isotactic poly(methyl methacrylate) and syndiotactic poly(methyl methacrylate)
In this paper, we present a different approach of coating a phase change material (PCM) for a possible use in thermal energy storage. We focused on layer-by-layer coating of pentaerythritol (PE) particles, whi...
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Article
Open AccessTreating the placenta to prevent adverse effects of gestational hypoxia on fetal brain development
Some neuropsychiatric disease, including schizophrenia, may originate during prenatal development, following periods of gestational hypoxia and placental oxidative stress. Here we investigated if gestational h...
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Article
Thermally resistant polylactide layer-by-layer film prepared using an inkjet approach
The inkjet printing technique is potentially applicable for the formation of polymer stereocomplexes and can control the amount, thickness and structure of the printed polymers. Stereocomplexes of polylactides...
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Chapter
Development of Full-Thickness Human Skin Equivalents with Blood and Lymph-like Capillary Networks by Cell Coating Technology
We developed a human skin equivalent (HSE) containing blood and lymph-like capillary networks using a cell-coating technique. This technique is a rapid fabrication technology of three-dimensional cellular cons...
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Article
Polymer design using trimethylene carbonate with ethylene glycol units for biomedical applications
Poly(trimethylene carbonate) (PTMC) is a biodegradable polymer that is used in biomedical materials. It has been reported that its combination with hydrophilic groups such as oligo(ethylene glycol) and poly(et...
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Article
Enhanced Thermal Stability of Polylactide by Terminal Conjugation Groups
Various acids such as aliphatic or carbocyclic fatty or aromatic acids were successfully conjugated into the ending hydroxyl group of poly(l-lactide) (PLLA). The chemical structures of various acid–PLLAs were con...
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Protocol
Nanomedicine and Infection
Nanomedicine is the medical application of nanotechnology and related study to the prevention and treatment of disease in the human body. In recent years, significant effort has been directed to develop nanote...
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Article
Interaction between living cells and polymeric particles: potential application of ionic liquid for evaluating the cellular uptake of biodegradable polymeric particles composed of poly(amino acid)
The cellular uptake of biodegradable particles as drug or vaccine carriers was observed by scanning electron microscopy employing an ionic liquid. The samples were observed by simply dip** them into the ioni...
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Article
Adsorption capability of urethane-crosslinked heptakis(2,6-di-O-methyl)-β-cyclodextrin polymers toward polychlorobiphenyls in nonpolar organic media
Urethane-crosslinked β-cyclodextrin polymers are prepared by crosslinking heptakis(2,6-di-O-methyl)-β-cyclodextrin (DM-β-CD) with various diisocyanate linkers, such as 4,4′-methylenebis(phenyl isocyanate) (MDI), ...
