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Article
Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts
Application of new genome editing technologies for plant improvement depends on efficient delivery of editing components into plant cells. For editing of canola, which is an important oil crop, CRISPR/Cas9 and Ag...
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Article
Open AccessDemonstration of targeted crossovers in hybrid maize using CRISPR technology
Naturally occurring chromosomal crossovers (CO) during meiosis are a key driver of genetic diversity. The ability to target CO at specific allelic loci in hybrid plants would provide an advantage to the plant ...
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Article
“Cell grafting”: a new approach for transferring cytoplasmic or nuclear genome between plants
A new method based on mixing and wounding of callus tissue was used to transfer plastid or nuclear DNA between cells.
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Article
Physical Characteristics of Kazan Minor Showers as Determined by Correlations with the Arecibo UHF Radar
In the northern hemisphere, the month of February is characterized by a lack of major meteor shower activity yet a number of weak minor showers are present as seen by the Kazan radar. Using the Feller transfor...
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Article
Agrobacterium-mediated transformation of seedling-derived maize callus
Efficient production of seedling-derived Type I callus was demonstrated for several corn genotypes including commercial inbred lines. Seeds were germinated on MS-based medium containing 10 mg l−1 picloram and 3 m...
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Article
Complementation in somatic hybrids indicates four types of nitrate reductase deficient lines in Nicotiana plumbaginifolia
Allelism of nine nitrate reductase deficient (NR−) Nicotiana plumbaginifolia cell lines was tested by complementation after protoplast fusion. Complementation was recognized by the appearance of somatic hybrid co...
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Article
Isoleucine-requiring Nicotiana plant deficient in threonine deaminase
Well characterized auxotrophic mutants would greatly assist the genetic manipulation of flowering plants, but such mutants are rare1–3. Moreover, auxotrophic mutants isolated at the plant level4–8 and some of tho...