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Article
Identification and validation of genes with expression patterns inverse to multiple metastasis suppressor genes in breast cancer cell lines
Metastasis suppressor genes (MSGs) have contributed to an understanding of regulatory pathways unique to the lethal metastatic process. When re-expressed in experimental models, MSGs block cancer spread to, an...
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Article
Open AccessIdentification of novel molecular regulators of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in breast cancer cells by RNAi screening
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) binds to its receptors, TRAIL-receptor 1 (TRAIL-R1) and TRAIL-receptor 2 (TRAIL-R2), leading to apoptosis by activation of caspase-8 and the down...
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Article
Open AccessCross-species genomic and functional analyses identify a combination therapy using a CHK1 inhibitor and a ribonucleotide reductase inhibitor to treat triple-negative breast cancer
Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer that is diagnosed in approximately 15% of all human breast cancer (BrCa) patients. Currently, no targeted therapies exist for this...
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Article
Open AccessSystems-wide RNAi analysis of CASP8AP2/FLASH shows transcriptional deregulation of the replication-dependent histone genes and extensive effects on the transcriptome of colorectal cancer cells
Colorectal carcinomas (CRC) carry massive genetic and transcriptional alterations that influence multiple cellular pathways. The study of proteins whose loss-of-function (LOF) alters the growth of CRC cells ca...
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Article
Identification of the receptor tyrosine kinase AXL in breast cancer as a target for the human miR-34a microRNA
The identification of molecular features that contribute to the progression of breast cancer can provide valuable insight into the pathogenesis of this disease. Deregulated microRNA expression represents one t...
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Article
Identification of WEE1 as a potential molecular target in cancer cells by RNAi screening of the human tyrosine kinome
Breast cancers can be classified into those that express the estrogen (ER) and progesterone (PR) receptors, those with ERBB2 (HER-2/Neu) amplification, and those without expression of ER, PR, or amplification of
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Article
Open AccessSpliceCenter: A suite of web-based bioinformatic applications for evaluating the impact of alternative splicing on RT-PCR, RNAi, microarray, and peptide-based studies
Over 60% of protein-coding genes in vertebrates express mRNAs that undergo alternative splicing. The resulting collection of transcript isoforms poses significant challenges for contemporary biological assays....
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Article
Open AccessPvt1-encoded microRNAs in oncogenesis
The functional significance of the Pvt1 locus in the oncogenesis of Burkitt's lymphoma and plasmacytomas has remained a puzzle. In these tumors, Pvt1 is the site of reciprocal translocations to immunoglobulin loc...
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Article
Open AccessInefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo
The cationic lipid Genzyme lipid (GL) 67 is the current "gold-standard" for in vivo lung gene transfer. Here, we assessed, if GL67 mediated uptake of siRNAs and asODNs into airway epithelium in vivo.
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Chapter
Gene Silencing through RNA Interference
A major focus of biology is determining the role that specific genes play within a cell. The ability to control gene expression is a powerful tool for biologists, and methods that facilitate the expression of ...
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Article
RNAi quashes polyQ
RNA interference in the brain inhibits neurodegeneration in a polyglutamine disease, SCA1. Is this now the way forward for the clinical treatment of certain genetic disorders (pages 816–820
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Article
Gene silencing through RNA interference: Potential for therapeutics and functional genomics
A major focus of biology is determining the role that specific genes play within a cell. The ability to control gene expression is a powerful tool for biologists, and methods that facilitate the expression of ...
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Protocol
Nucleic Acid Transfer Using Cationic Lipids
The use of cationic lipids or cationic polymers to mediate the transfer of nucleic acids into mammalian cells has become a widely applied technology in recent years. The principal reasons for this have been th...
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Chapter and Conference Paper
Lipid Gene Trasfer, a Story of Simplicity and Complexity
It is 10 years since the first description of the use of a positively charged lipid to deliver nucleic acids to mammalian cells (Feigner et al., 1987). In essence the methodology of liposome-mediated transfer of ...
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Chapter and Conference Paper
Gene Therapy for Cancer
Cancer is a complex acquired disease that affects millions of individuals each year. Underlying the pathogenesis of cancer are a variety of molecular genetic abnormalities which can be inherited or environment...
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Chapter and Conference Paper
Lipid Gene Transfer and Clinical Gene Therapy
There are several features of liposome-mediated DNA transfer that make it a viable delivery system for clinical gene therapy. 1) In contrast to viral delivery systems, contamination with exogenous protein or o...
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Article
Book reviews
An Introduction to Recombinant DNA in Medicine (2nd edn).
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Article
Liposome-mediated CFTR gene transfer to the nasal epithelium of patients with cystic fibrosis
We report the results of a double-blind, placebo-controlled trial in nine cystic fibrosis (CF) subjects receiving cationic liposome complexed with a complementary DNA encoding the CF transmembrane conductance ...
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Article
Complement C4 and heat shock protein 70 (HSP70) genotypes and type I diabetes mellitus
Type I diabetes is strongly associated with the major histocompatibility complex (MHC) class II region (DR and DQ loci), and to a lesser extent the class III region (complement C4 loci). Restriction fragment leng...