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Gene Silencing through RNA Interference

Potential for Therapeutics and Functional Genomics

  • Chapter
Peptide Nucleic Acids, Morpholinos and Related Antisense Biomolecules

Part of the book series: Medical Intelligence Unit ((MIUN))

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Abstract

A major focus of biology is determining the role that specific genes play within a cell. The ability to control gene expression is a powerful tool for biologists, and methods that facilitate the expression of genes in trans have been very useful in delineating gene function. However, methods that enable researchers to decrease or inhibit gene expression in a controlled and specific manner have been lacking. A significant advancement in the field of gene silencing is the recent discovery of RNA interference (RNAi), a post-transcriptional gene silencing mechanism first described in C. elegans and Drosophila and more recently shown to occur in mammals, including mouse and human cells. This chapter will examine several aspects of RNAi and its related gene silencing mechanisms and will discuss the impact that technologies based on these endogenous cellular processes have had on studies of gene function and on new approaches to the treatment of disease.

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Authors and Affiliations

  1. Translational Genomics Research Institute (TGen), Gaithersburg, Maryland, USA

    David O. Azorsa & Spyro Mousses

  2. Medical Genetics Branch National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA

    Natasha J. Caplen

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  1. David O. Azorsa
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Azorsa, D.O., Mousses, S., Caplen, N.J. (2006). Gene Silencing through RNA Interference. In: Peptide Nucleic Acids, Morpholinos and Related Antisense Biomolecules. Medical Intelligence Unit. Springer, Boston, MA. https://doi.org/10.1007/0-387-32956-0_17

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