High-Throughput RNAi Screening
Methods and Protocols
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Open AccessIn vitro activity of a G-quadruplex-stabilizing small molecule that synergizes with Navitoclax to induce cytotoxicity in acute myeloid leukemia cells
Acute Myeloid Leukemia (AML) is a malignancy of myeloid precursor cells that arise from genomic alterations in the expression of key growth regulatory genes causing cells to assume an undifferentiated state an...
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Open AccessDisulfiram overcomes bortezomib and cytarabine resistance in Down-syndrome-associated acute myeloid leukemia cells
Children with Down syndrome (DS) have increased risk for develo** AML (DS-AMKL), and they usually experience severe therapy-related toxicities compared to non DS-AMKL. Refractory/relapsed disease has very po...
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Protocol
High-Throughput RNAi Screening for the Identification of Novel Targets
Gene silencing through RNA interference has provided researchers with an effective way to study gene function. High-throughput RNA interference (HT-RNAi) screening has further permitted researchers to identify...
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Article
Open AccessRNAi phenotype profiling of kinases identifies potential therapeutic targets in Ewing's sarcoma
Ewing's sarcomas are aggressive musculoskeletal tumors occurring most frequently in the long and flat bones as a solitary lesion mostly during the teen-age years of life. With current treatments, significant n...
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Open AccessHigh-content siRNA screening of the kinome identifies kinases involved in Alzheimer's disease-related tau hyperphosphorylation
Neurofibrillary tangles (NFT), a cardinal neuropathological feature of Alzheimer's disease (AD) that is highly correlated with synaptic loss and dementia severity, appear to be partly attributable to increased...
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Open AccessSynthetic lethal RNAi screening identifies sensitizing targets for gemcitabine therapy in pancreatic cancer
Pancreatic cancer retains a poor prognosis among the gastrointestinal cancers. It affects 230,000 individuals worldwide, has a very high mortality rate, and remains one of the most challenging malignancies to ...
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Chapter
Gene Silencing through RNA Interference
A major focus of biology is determining the role that specific genes play within a cell. The ability to control gene expression is a powerful tool for biologists, and methods that facilitate the expression of ...
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Article
Nonsense-mediated decay microarray analysis identifies mutations of EPHB2 in human prostate cancer
The identification of tumor-suppressor genes in solid tumors by classical cancer genetics methods is difficult and slow. We combined nonsense-mediated RNA decay microarrays1 and array-based comparative genomic hy...
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Open AccessPhenotypic alterations in breast cancer cells overexpressing the nuclear receptor co-activator AIB1
Estrogen signaling plays a critical role in a number of normal physiological processes and has important implications in the treatment of breast cancer. The p160 nuclear receptor coactivator, AIB1 (amplified in b...
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Article
Gene silencing through RNA interference: Potential for therapeutics and functional genomics
A major focus of biology is determining the role that specific genes play within a cell. The ability to control gene expression is a powerful tool for biologists, and methods that facilitate the expression of ...
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Article
Association of steroid receptor coactivator AIB1 with estrogen receptor-α in breast cancer cells
The steroid receptor coactivator AIB1 (amplified in breast cancer-1) is a transcriptional coactivator which has been found to be amplified in breast cancer. We have now investigated the role of the AIB1 protei...
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cDNA microarrays detect activation of a myogenic transcription program by the PAX3-FKHR fusion oncogene
