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Synthesis of hollow magnetic carbon microbeads using iron oleate@alginate core-shell hydrogels and their application to magnetic separation of organic dye
The use of biopolymers obtained from natural resources as a carbon source has attracted much attention. In this study, we introduced a novel method...
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A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level
Protein secretion is a key cellular functionality, particularly in immunology, where cells can display large heterogeneity in this crucial activity...
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Double Emulsion Flow Cytometry for Rapid Single Genome Detection
Established techniques in droplet microfluidics have utilized single emulsion (SE) drops to compartmentalize and analyze single cells achieving...
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Comparison of HPLC–DAD and LC–MS Techniques for the Determination of Tetracyclines in Medicated Feeds Using One Extraction Protocol
Four antibiotics, oxytetracycline, tetracycline, doxycycline, and chlortetracycline were separated and quantified in medicated feed. Tetracyclines...
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Single-Drop Microextraction
Since its discovery in 1995 by Dasgupta et al., and further implementation by Jeannot et al. in 1996, single-drop microextraction (SDME) has...
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DNA Sizing with pg/ÎĽL Sensitivity for Cell-Free DNA Analysis with the ÎĽLAS Technology
The ÎĽLAS technology enables in-line DNA concentration and separation in a microchannel. Here, we describe its operation to analyze the size profile...
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Hollow-Fibre Liquid-Phase Microextraction
This chapter discusses microextraction using liquid membranes immobilized in a porous support membrane, including two- and three-phase hollow fibre...
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Scope of Three-Dimensional Printing for Fabrication of Foods
Three–dimensional (3D) printing has been receiving prime focus for the production of customized foods by accurately balancing both palatability and...
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Isolation of Bacteriophages on Actinobacteria Hosts
Bacteriophages are ubiquitous biological entities which can be found in a variety of habitats. Here, we describe protocols for the isolation of...
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Overexpression and Purification of Mitogenic and Metabolic Fibroblast Growth Factors
Fibroblast growth factors (FGFs) are proteins with a vast array of biological activity, such as cell development and repair, glucose and bile acid...
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Fabrication of Electrospun PVA-Aloe Vera Hybrid Nanofibers: Dye Removal Ability from Wastewater
Hybrid of polyvinyl alcohol and aloe vera gel (PVA@AVG) electrospun nanofiber composite has been developed by the electrospinning technique. AVG was...
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Identifying Ice-Binding Proteins in Nature
Organisms inhabiting freezing terrestrial, polar, and alpine environments survive because they have evolved adaptations to tolerate sub-freezing...
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Portable Solar Stirling Engine
Solar-based dish-Stirling system has ended up being the most proficient method for creating power utilizing sun-based energy. Because of the...
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Nanoliter-Scale Sample Preparation for Single-Cell Proteomic Analysis Using Glass-Oil-Air-Droplet Chip
Single-cell proteomic analyses are of fundamental importance in order to capture biological heterogeneity within complex cell systems’ heterogeneous...
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Degradation of Chlorpyrifos Pesticide Using a Postbiotic Enzyme of Lactic Acid Bacteria
Lactic acid bacteria (LAB) are well known for their production of postbiotic components such as organic acids, short-chain fatty acids, enzymes,...
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Sample Preparation for Metabolite Detection in Mass Spectrometry Imaging
Metabolites reflect the biological state of cells and tissue, and metabolomics is therefore a field of high interest both to understand normal...
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Enhancing the Efficiency of Conventional Surface Immunoassays Within Standard Labware Using Microscale Flows
In this chapter, we present the design and fabrication of a device and implementation of a protocol to realize increased efficiency of immunoassays...
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Matrix Effects Free Imaging of Thin Tissue Sections Using Pneumatically Assisted Nano-DESI MSI
Mass spectrometry imaging has the potential to reveal important molecular interaction in morphological regions in tissue. However, the simultaneous...
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Update DESI Mass Spectrometry Imaging (MSI)
Desorption electrospray ionization (DESI) is an ambient technique that allows chemical information to be obtained directly from a wide range of...
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Generation of Red Blood Cell Nanovesicles as a Delivery Tool
Extracellular vesicles (EVs) are natural membranous vesicles with immense potential as drug delivery tools. However, their large-scale production...