Abstract
Mass spectrometry imaging has the potential to reveal important molecular interaction in morphological regions in tissue. However, the simultaneous ionization of the continuously altered and complex chemistry in each pixel can introduce artifacts that result in skewed molecular distributions in the compiled ion images. These artifacts are known as matrix effects. Mass spectrometry imaging using nanospray desorption electrospray ionization (nano-DESI MSI) enables the elimination of matrix effects by do** the nano-DESI solvent with internal standards. Carefully selected internal standards ionize similarly and simultaneously with the extracted analytes from thin tissue sections, and the matrix effects are eliminated through a robust data normalization method. Herein we describe the setup and use of pneumatically assisted (PA) nano-DESI MSI with standards doped in the solvent for elimination of matrix effects in ion images.
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References
Roach PJ, Laskin J, Laskin A (2010) Nanospray desorption electrospray ionization: An ambient method for liquid-extraction surface sampling in mass spectrometry. Analyst 135:2233–2236. https://doi.org/10.1039/c0an00312c
Laskin J, Heath BS, Roach PJ et al (2012) Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry. Anal Chem 46:141–148. https://doi.org/10.1021/ac2021322
Nguyen SN, Sontag RL, Carson JP et al (2018) Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy. J Am Soc Mass Spectrom 29:316–322. https://doi.org/10.1007/s13361-017-1750-8
Lanekoff I, Thomas M, Carson JP et al (2013) Imaging nicotine in rat brain tissue by use of nanospray desorption electrospray ionization mass spectrometry. Anal Chem 85:882–889. https://doi.org/10.1021/ac302308p
Lanekoff I, Heath BS, Liyu A et al (2012) Automated platform for high-resolution tissue imaging using nanospray desorption electrospray ionization mass spectrometry. Anal Chem 84:8351–8356. https://doi.org/10.1021/ac301909a
Vandergrift GW, Kew W, Lukowski JK et al (2022) Imaging and Direct Sampling Capabilities of Nanospray Desorption Electrospray Ionization with Absorption-Mode 21 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Anal Chem 94:3629–3636. https://doi.org/10.1021/acs.analchem.1c05216
Sisley EK, Hale OJ, Styles IB, Cooper HJ (2022) Native Ambient Mass Spectrometry Imaging of Ligand-Bound and Metal-Bound Proteins in Rat Brain. J Am Chem Soc 144:2120–2128. https://doi.org/10.1021/jacs.1c10032
Hsu CC, Chou PT, Zare RN (2015) Imaging of Proteins in Tissue Samples Using Nanospray Desorption Electrospray Ionization Mass Spectrometry. Anal Chem 87:11171–11175. https://doi.org/10.1021/acs.analchem.5b03389
Duncan KD, Bergman HM, Lanekoff I (2017) A pneumatically assisted nanospray desorption electrospray ionization source for increased solvent versatility and enhanced metabolite detection from tissue. Analyst 142:3424–3431. https://doi.org/10.1039/c7an00901a
Santos VG, Regiani T, Dias FFG et al (2011) Venturi easy ambient sonic-spray ionization. Anal Chem 83:1375–1380. https://doi.org/10.1021/ac102765z
Lillja J, Duncan KD, Lanekoff I (2020) Determination of Monounsaturated Fatty Acid Isomers in Biological Systems by Modeling MS3 Product Ion Patterns. J Am Soc Mass Spectrom 31:2479–2487. https://doi.org/10.1021/jasms.0c00194
Duncan KD, Fang R, Yuan J et al (2018) Quantitative Mass Spectrometry Imaging of Prostaglandins as Silver Ion Adducts with Nanospray Desorption Electrospray Ionization. Anal Chem 90:7246–7252. https://doi.org/10.1021/acs.analchem.8b00350
Unsihuay D, Su P, Hu H et al (2021) Imaging and Analysis of Isomeric Unsaturated Lipids through Online Photochemical Derivatization of Carbon–Carbon Double Bonds. Angew Chemie Int Ed 60:7559–7563. https://doi.org/10.1002/anie.202016734
Chen CL, Kuo TH, Chung HH et al (2021) Remodeling nanoDESI Platform with Ion Mobility Spectrometry to Expand Protein Coverage in Cancerous Tissue. J Am Soc Mass Spectrom 32:653–660. https://doi.org/10.1021/jasms.0c00354
Lanekoff I, Cha J, Kyle JE et al (2016) Trp53 deficient mice predisposed to preterm birth display region-specific lipid alterations at the embryo implantation site. Sci Rep 6:1–9. https://doi.org/10.3390/ijms19113292
Duncan KD, Sun X, Baker ES et al (2021) In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases. Commun Biol 4:1–9. https://doi.org/10.1038/s42003-021-02488-1
Mavroudakis L, Stevens SL, Duncan KD, et al. (2020) CpG preconditioning reduces accumulation of lysophosphatidylcholine in ischemic brain tissue after middle cerebral artery occlusion
Bergman HM, Lindfors L, Palm F et al (2019) Metabolite aberrations in early diabetes detected in rat kidney using mass spectrometry imaging. Anal Bioanal Chem 411:2809–2816. https://doi.org/10.1007/s00216-019-01721-5
Mavroudakis L, Duncan KD, Lanekoff I (2022) Host-Guest Chemistry for Simultaneous Imaging of Endogenous Alkali Metals and Metabolites with Mass Spectrometry. Anal Chem 94:2391–2398. https://doi.org/10.1021/acs.analchem.1c03913
Lanekoff I, Stevens SL, Stenzel-Poore MP, Laskin J (2014) Matrix effects in biological mass spectrometry imaging: identification and compensation. Analyst 139:3528–3532. https://doi.org/10.1039/c4an00504j
Nguyen SN, Kyle JE, Dautel S, et al. (2019) Lipid coverage in nanospray desorption electrospray ionization mass spectrometry imaging of mouse lung tissues. Anal Chem acs.analchem.9b02045. https://doi.org/10.1021/acs.analchem.9b02045
Lanekoff I, Laskin J (2015) Imaging of Lipids and Metabolites Using Nanospray Desorption Electrospray Ionization Mass Spectrometry. In: He L (ed) Mass Spectrometry Imaging of Small Molecules. Springer New York, New York, NY, pp 99–106
Lanekoff I, Thomas M, Laskin J (2014) Shotgun approach for quantitative imaging of phospholipids using nanospray desorption electrospray ionization mass spectrometry. Anal Chem 86:1872–1880. https://doi.org/10.1021/ac403931r
Yin R, Kyle J, Burnum-Johnson K et al (2018) High Spatial Resolution Imaging of Mouse Pancreatic Islets Using Nanospray Desorption Electrospray Ionization Mass Spectrometry. Anal Chem 90:6548–6555. https://doi.org/10.1021/acs.analchem.8b00161
Duncan KD, Lanekoff I (2018) Oversampling to Improve Spatial Resolution for Liquid Extraction Mass Spectrometry Imaging. Anal Chem 90:2451–2455. https://doi.org/10.1021/acs.analchem.7b04687
Acknowledgments
Funding for this protocol was provided through projects funded by the Swedish Foundation for Strategic Research (SSF, ITM17-0014) and the Swedish Research Council (VR, 2017-04125).
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Mavroudakis, L., Lanekoff, I. (2023). Matrix Effects Free Imaging of Thin Tissue Sections Using Pneumatically Assisted Nano-DESI MSI. In: Cole, L.M., Clench, M.R. (eds) Imaging Mass Spectrometry. Methods in Molecular Biology, vol 2688. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3319-9_10
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DOI: https://doi.org/10.1007/978-1-0716-3319-9_10
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