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Enhancing the Efficiency of Conventional Surface Immunoassays Within Standard Labware Using Microscale Flows

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Microfluidics Diagnostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2804))

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Abstract

In this chapter, we present the design and fabrication of a device and implementation of a protocol to realize increased efficiency of immunoassays within microtiter plates. The device, WellProbe, is a 3D-structured probe that can be used to deliver precise flows at the bottom of standard well plates to establish concentric areas of shear stress intensities using hydrodynamically confined flows. The protocols involve both operation and data analysis.

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References

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Author information

Authors and Affiliations

  1. IBM Research Europe, Rüschlikon, Switzerland

    Iago Pereiro, Anna Fomitcheva-Khartchenko & Govind V. Kaigala

  2. School of Biomedical Engineering, Vancouver Prostate Center, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada

    Govind V. Kaigala

Authors
  1. Iago Pereiro
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  2. Anna Fomitcheva-Khartchenko
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  3. Govind V. Kaigala
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Corresponding authors

Correspondence to Iago Pereiro or Govind V. Kaigala .

Editor information

Editors and Affiliations

  1. Centre de recherche des Cordeliers, INSERM, UMR-S1138, CNRS SNC 5096, Université Paris Cité, Paris, Paris, France

    Valérie Taly

  2. UMR 168, Institut Pierre-Gilles de Gennes, Institut Curie, Paris, Paris, France

    Stéphanie Descroix

  3. CEA, INRAE, Médicaments et Technologies pour la Santé (MTS), SPI, Université Paris-Saclay, Gif-sur-Yvette, Paris, France

    Karla Perez-Toralla

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Pereiro, I., Fomitcheva-Khartchenko, A., Kaigala, G.V. (2024). Enhancing the Efficiency of Conventional Surface Immunoassays Within Standard Labware Using Microscale Flows. In: Taly, V., Descroix, S., Perez-Toralla, K. (eds) Microfluidics Diagnostics. Methods in Molecular Biology, vol 2804. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3850-7_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3850-7_6

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3849-1

  • Online ISBN: 978-1-0716-3850-7

  • eBook Packages: Springer Protocols

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